CCL15 Antibody | MyBioSource MBS9209414 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CCL15 Antibody

catalog :

MBS9209414

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, flow cytometry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Overlay histogram showing MCF-7 cells stained with MBS9209414 (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody ( , 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1Ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

image 2 :

Anti-CCL15 Antibody at 1:1000 dilution + human heart lysates Lysates/proteins at 20 Ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 12 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

image 3 :

Anti-CCL15 Antibody at 1:1000 dilution + human heart lysates. Lysates/proteins at 20 ug per lane. Secondary. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 12 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

product information

catalog number :

MBS9209414

products type :

Antibody

products full name :

CCL15 Antibody

products short name :

[CCL15]

products name syn :

[C-C motif chemokine 15; Chemokine CC-2; HCC-2; Leukotactin-1; LKN-1; MIP-1 delta; Macrophage inflammatory protein 5; MIP-5; Mrp-2b; NCC-3; Small-inducible cytokine A15; CCL15(22-92); CCL15(25-92); CCL15(29-92); CCL15; MIP5; NCC3; SCYA15]

other names :

[C-C motif chemokine 15 preproprotein; C-C motif chemokine 15; C-C motif chemokine 15; chemokine (C-C motif) ligand 15; Chemokine CC-2; HCC-2]

products gene name :

[CCL15]

other gene names :

[CCL15; CCL15; LKN1; NCC3; SY15; HCC-2; LKN-1; MIP-5; NCC-3; SCYL3; MIP-1D; MRP-2B; SCYA15; HMRP-2B; MIP-1 delta; MIP5; NCC3; SCYA15; HCC-2; LKN-1; MIP-5]

uniprot entry name :

CCL15_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human

sequence length :

113

specificity :

This CCL15 antibody is generated from a rabbit immunized with a recombinant protein.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC)

app notes :

FC~~1:25. IHC~~1:25. WB~~1:2000

image1 heading :

Flow Cytometry

image2 heading :

Immunohistochemistry

image3 heading :

Western Blot (WB)

image4 heading :

Western Blot (WB)

image4 description :

Anti-CCL15 Antibody at 1:2000 dilution + human liver lysates. Lysates/proteins at 20 ug per lane. Secondary. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 12 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

other info1 :

Crown Antibody: Yes. Antigen Region: 1-113

other info2 :

Antigen Source: HUMAN. Function: Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the small-inducible cytokine A15. Cellular Location: Secreted. Tissue Location: Most abundant in heart, skeletal muscle and adrenal gland. Lower levels in placenta, liver, pancreas and bone marrow. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are found in high levels in synovial fluids from rheumatoid patients

products categories :

Crown Antibodies

products description :

Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the small-inducible cytokine A15.

products references :

Youn B.-S.,et al.J. Immunol. 159:5201-5205(1997). Wang W.,et al.J. Clin. Immunol. 18:214-222(1998). Pardigol A.,et al.Proc. Natl. Acad. Sci. U.S.A. 95:6308-6313(1998). Nomiyama H.,et al.J. Interferon Cytokine Res. 19:227-234(1999). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

ncbi gi num :

597709818

ncbi acc num :

NP_116741.2

ncbi gb acc num :

NM_032965.5

uniprot acc num :

Q16663

ncbi mol weight :

12248

ncbi pathways :

Phosphonate And Phosphinate Metabolism Pathway (6359); Phosphonate And Phosphinate Metabolism Pathway (6359); Phosphonate And Phosphinate Metabolism Pathway (6359); Phosphonate And Phosphinate Metabolism Pathway (6359)

ncbi summary :

This gene is located in a cluster of similar genes in the same region of chromosome 17. These genes encode CC cytokines, which are secreted proteins characterized by two adjacent cysteines. The product of this gene is chemotactic for T cells and monocytes, and acts through C-C chemokine receptor type 1 (CCR1). The proprotein is further processed into numerous smaller functional peptides. Naturally-occurring readthrough transcripts occur from this gene into the downstream gene, CCL14 (chemokine (C-C motif) ligand 14). [provided by RefSeq, Jan 2013]

uniprot summary :

CCL15: Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the small-inducible cytokine A15. Belongs to the intercrine beta (chemokine CC) family. Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 17q12. Cellular Component: extracellular space. Molecular Function: heparin binding; chemokine activity; receptor binding; chemoattractant activity. Biological Process: cellular calcium ion homeostasis; cell-cell signaling; positive chemotaxis; immune response; signal transduction; chemotaxis

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage