ROR1 Antibody (N-term) | MyBioSource MBS9209314 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ROR1 Antibody (N-term)

catalog :

MBS9209314

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of ROR1 (arrow) using rabbit polyclonal ROR1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ROR1 gene (Lane 2) (Origene Technologies).

image 2 :

Flow cytometric analysis of NCI-H460 cells using ROR1 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

product information

catalog number :

MBS9209314

products type :

Antibody

products full name :

ROR1 Antibody (N-term)

products short name :

[ROR1]

products name syn :

[Tyrosine-protein kinase transmembrane receptor ROR1; Neurotrophic tyrosine kinase; receptor-related 1; ROR1; NTRKR1]

other names :

[tyrosine-protein kinase transmembrane receptor ROR1 isoform 2; Tyrosine-protein kinase transmembrane receptor ROR1; tyrosine-protein kinase transmembrane receptor ROR1; receptor tyrosine kinase-like orphan receptor 1; Neurotrophic tyrosine kinase, receptor-related 1]

products gene name :

[ROR1]

other gene names :

[ROR1; ROR1; NTRKR1; dJ537F10.1; NTRKR1]

uniprot entry name :

ROR1_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human

sequence positions :

[32-62]

sequence length :

393

specificity :

This ROR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-62 amino acids from the N-terminal region of human ROR1.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

concentration :

Vial Concentration: 2

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)

app notes :

WB~~1:1000. FC~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Flow Cytometry (FC/FACS)

other info1 :

Antigen Type: Synthetic Peptide

other info2 :

Antigen Source: HUMAN. Function: Has very low kinase activity in vitro and is unlikely to function as a tyrosine kinase in vivo. May act as a receptor for wnt ligand WNT5A which may result in the inhibition of WNT3A- mediated signaling. Cellular Location: Membrane; Single-pass type I membrane protein. Tissue Location: Expressed strongly in human heart, lung and kidney, but weakly in the CNS. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm. ROR1 Antibody (N-term) - Protocol: Provided below are standard protocols that you may find useful for product applications. ROR subfamily of cell surface receptors. Studies of a similar protein in mouse suggest that this protein may interact with another receptor protein tyrosine kinase and may be involved in skeletal and cardiac development.

products categories :

Neuroscience; Signal Transduction

products description :

ROR1 is a receptor protein tyrosine kinase whose cellular role has not been determined. It is a type I membrane protein and belongs to the ROR subfamily of cell surface receptors. Studies of a similar protein in mouse suggest that this protein may interact with another receptor protein tyrosine kinase and may be involved in skeletal and cardiac development.

products references :

Nomi, M., et al., Mol. Cell. Biol. 21(24):8329-8335 (2001). Reddy, U.R., et al., Genomics 41(2):283-285 (1997). Reddy, U.R., et al., Oncogene 13(7):1555-1559 (1996). Masiakowski, P., et al., J. Biol. Chem. 267(36):26181-26190 (1992).

ncbi gi num :

134152686

ncbi acc num :

NP_001077061.1

ncbi gb acc num :

NM_001083592.1

uniprot acc num :

Q01973

ncbi mol weight :

104283

ncbi pathways :

Butanoate Metabolism Pathway (4919); Butanoate Metabolism Pathway (4919); Butanoate Metabolism Pathway (4919); Butanoate Metabolism Pathway (4919); Butanoate Metabolism Pathway (4919); Butanoate Metabolism Pathway (4919)

ncbi summary :

This gene encodes a receptor tyrosine kinase-like orphan receptor that modulates neurite growth in the central nervous system. The encoded protein is a glycosylated type I membrane protein that belongs to the ROR subfamily of cell surface receptors. It is a pseudokinase that lacks catalytic activity and may interact with the non-canonical Wnt signalling pathway. This gene is highly expressed during early embryonic development but expressed at very low levels in adult tissues. Increased expression of this gene is associated with B-cell chronic lymphocytic leukaemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2012]

uniprot summary :

ROR1: Tyrosine-protein kinase receptor whose role is not yet clear. Expressed strongly in human heart, lung and kidney, but weakly in the CNS. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm. Belongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Protein kinase, TK; Kinase, protein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; Ror family. Chromosomal Location of Human Ortholog: 1p31.3. Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; receptor complex. Molecular Function: Wnt-protein binding; protein binding; transmembrane receptor protein tyrosine kinase activity; ATP binding. Biological Process: peptidyl-tyrosine phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage