ELISA/assay

Human DNA-binding protein Ikaros, IKZF1 ELISA Kit

MBS920900

48-Strip-Wells

510 USD

ELISA Kit

Human DNA-binding protein Ikaros, IKZF1 ELISA Kit

IKAROS family zinc finger 1 (Ikaros)

Human DNA-binding protein Ikaros (IKZF1) ELISA kit; Hs.54452; IK1; IKAROS; LYF1; PRO0758; ZNFN1A1; hIk-1; CLL-associated antigen KW-6; Ikaros (zinc finger protein) ; zinc finger protein; subfamily 1A; 1 (Ikaros) ; IKAROS family zinc finger 1 (Ikaros)

DNA-binding protein Ikaros isoform 2; DNA-binding protein Ikaros; DNA-binding protein Ikaros; CLL-associated antigen KW-6; ikaros family zinc finger protein 1; lymphoid transcription factor LyF-1; protein phosphatase 1, regulatory subunit 92; zinc finger protein, subfamily 1A, 1 (Ikaros); IKAROS family zinc finger 1 (Ikaros); Ikaros family zinc finger protein 1; Lymphoid transcription factor LyF-1

IKZF1

IKZF1; IKZF1; IK1; LYF1; LyF-1; IKAROS; PPP1R92; PRO0758; ZNFN1A1; Hs.54452; IK1; IKAROS; LYF1; ZNFN1A1

IKZF1_HUMAN

Human

477

This assay has high sensitivity and excellent specificity for detection of human IKZF1. No significant cross-reactivity or interference between human IKZF1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: 7.8 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IKZF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IKZF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IKZF1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IKZF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

334085209

NP_001207694.1

NM_001220765.2

Q13422

57,528 Da

Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993)

This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors. Overexpression of some dominant-negative isoforms have been associated with B-cell malignancies, such as acute lymphoblastic leukemia (ALL). [provided by RefSeq, May 2014]

Ikaros: a transcription factor of the ikaros C2H2-type zinc-finger protein family. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Functions in the specification and the maturation of the T-lymphocyte. Also interacts with a critical control element in the TDT (terminal deoxynucleotidyltransferase) promoter as well as with the promoters for other genes expressed during early stages of B- and T-cell development. Deletions in Ikaros have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Seven alternatively spliced human isoforms have been described. Protein type: Transcription factor; C2H2-type zinc finger protein; DNA-binding. Chromosomal Location of Human Ortholog: 7p12.2. Cellular Component: centric heterochromatin; protein complex; cytoplasm; nucleus. Molecular Function: DNA binding; protein heterodimerization activity; sequence-specific DNA binding; metal ion binding; transcription factor activity. Biological Process: Peyer s patch development; transcription, DNA-dependent; thymus development; chromatin modification; positive regulation of multicellular organism growth; negative regulation of transcription from RNA polymerase II promoter; cell cycle; lymph node development; positive regulation of neutrophil differentiation; retina development in camera-type eye; natural killer cell differentiation; B cell differentiation; forebrain development; mesoderm development; positive regulation of NK T cell differentiation; positive regulation of transcription from RNA polymerase II promoter; T cell differentiation

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800