Mouse Cdk9 Antibody (C-term) | MyBioSource MBS9208988 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Cdk9 Antibody (C-term)

catalog :

MBS9208988

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of lysates from 293, mouse NIH/3T3, rat C6 cell line and mouse kidney tissue lysate(from left to right), using Mouse Cdk9 Antibody (C-term). MBS9208988 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

product information

catalog number :

MBS9208988

products type :

Antibody

products full name :

Mouse Cdk9 Antibody (C-term)

products short name :

[Cdk9]

products name syn :

[Cyclin-dependent kinase 9; Cell division protein kinase 9; Cdk9]

other names :

[cyclin-dependent kinase 9; Cyclin-dependent kinase 9; cyclin-dependent kinase 9; cyclin-dependent kinase 9 (CDC2-related kinase); Cell division protein kinase 9]

products gene name :

[Cdk9]

other gene names :

[Cdk9; Cdk9; PITALRE]

uniprot entry name :

CDK9_MOUSE

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human, mouse (Predicted Reactivity: Bovine, Rat)

sequence positions :

[251-278]

sequence length :

372

specificity :

This Mouse Cdk9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 251-278 amino acids from the C-terminal region of mouse Cdk9.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA)

app notes :

WB~~1:1000

image1 heading :

Western Blot (WB)

other info1 :

Function: Protein kinase involved in the regulation of transcription. Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A, SUPT5H and RDBP. This complex is inactive when in the 7SK snRNP complex form. Phosphorylates EP300, MYOD1, RPB1/POLR2A and AR, and the negative elongation factors DSIF and NELF. Regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors (e.g. TNF-inducible RELA/p65 activation and IL-6-inducible STAT3 signaling). Promotes RNA synthesis in genetic programs for cell growth, differentiation and viral pathogenesis. P-TEFb is also involved in cotranscriptional histone modification, mRNA processing and mRNA export. Modulates a complex network of chromatin modifications including histone H2B monoubiquitination (H2Bub1), H3 lysine 4 trimethylation (H3K4me3) and H3K36me3; integrates phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing. The CDK9/cyclin-K complex has also a kinase activity towards CTD of RNAP II and can substitute for CDK9/cyclin-T P-TEFb in vitro. Replication stress response protein; the CDK9/cyclin-K complex is required for genome integrity maintenance, by promoting cell cycle recovery from replication arrest and limiting single- stranded DNA amount in response to replication stress, thus reducing the breakdown of stalled replication forks and avoiding DNA damage. In addition, probable function in DNA repair of isoform 2 via interaction with KU70/XRCC6. Promotes cardiac myocyte enlargement. RPB1/POLR2A phosphorylation on 'Ser-2' in CTD activates transcription. AR phosphorylation modulates AR transcription factor promoter selectivity and cell growth. DSIF and NELF phosphorylation promotes transcription by inhibiting their negative effect. The phosphorylation of MYOD1 enhances its transcriptional activity and thus promotes muscle differentiation (By similarity).

other info2 :

Cellular Location: Nucleus. Cytoplasm. Nucleus, PML body Note=Accumulates on chromatin in response to replication stress Complexed with CCNT1 in nuclear speckles, but uncomplexed form in the cytoplasm. The translocation from nucleus to cytoplasm is XPO1/CRM1-dependent. Associates with PML body when acetylated (By similarity). Tissue Location: Expressed at high levels in brain and kidney

products categories :

Crown Antibodies; Cell Biology; Immunology; Neuroscience; Signal Transduction

products description :

Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro (By similarity).

products references :

Yokoyama, S., et al. Dev. Cell 17(6):836-848(2009). Alarcon, C., et al. Cell 139(4):757-769(2009). Takaya, T., et al. Circ. J. 73(8):1492-1497(2009). Kohoutek, J., et al. Mol. Cell. Biol. 29(12):3280-3285(2009). Elagib, K.E., et al. Blood 112(13):4884-4894(2008)

ncbi gi num :

18699998

ncbi acc num :

NP_570930.1

ncbi gb acc num :

NM_130860.3

uniprot acc num :

Q99J95

ncbi mol weight :

42762

ncbi pathways :

, Pathway (107951); , Pathway (107951); , Pathway (107951); , Pathway (107951); , Pathway (107951); , Pathway (107951); , Pathway (107951); , Pathway (107951); , Pathway (107951); , Pathway (107951)

uniprot summary :

CDK9: a protein kinase of the CDK family. Forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. A component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. Transcriptional elongation factor and cofactor for HIV Tat protein; RNAi blocks HIV replication, and inhibitors also block varicella zoster replication. Mediates signals leading to cardiac hypertrophy. Inhibitor: Flavopiridol. Protein type: EC 2.7.11.22; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; EC 2.7.11.23; Nuclear receptor co-regulator; Cell cycle regulation; CMGC group; CDK family; CDK9 subfamily; CDK/CDK9 subfamily. Cellular Component: nucleoplasm; transcription elongation factor complex; PML body; transcription elongation factor complex b; membrane; intracellular membrane-bound organelle; cytoplasm; nucleus. Molecular Function: nucleotide binding; transcription factor binding; protein kinase activity; RNA polymerase subunit kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; snRNA binding; DNA binding; cyclin-dependent protein kinase activity; transferase activity, transferring phosphorus-containing groups; chromatin binding; kinase activity; ATP binding. Biological Process: regulation of DNA repair; regulation of histone modification; positive regulation of histone phosphorylation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; replication fork arrest; DNA repair; protein amino acid phosphorylation; response to DNA damage stimulus; phosphorylation

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage