product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human Ketohexokinase, KHK ELISA Kit
catalog :
MBS920892
quantity :
96 Strip Wells
price :
760 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS920892
products type :
ELISA Kit
products full name :
Human Ketohexokinase, KHK ELISA Kit
products short name :
ketohexokinase (fructokinase)
products name syn :
Human Ketohexokinase (KHK) ELISA kit; ketohexokinase; ketohexokinase (fructokinase)
other names :
ketohexokinase isoform a; Ketohexokinase; ketohexokinase; hepatic fructokinase; ketohexokinase (fructokinase); Hepatic fructokinase
products gene name :
KHK
other gene names :
KHK; KHK
uniprot entry name :
KHK_HUMAN
reactivity :
Human
sequence length :
298
specificity :
This assay has high sensitivity and excellent specificity for detection of human KHK. No significant cross-reactivity or interference between human KHK and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: For the quantitative determination of human ketohexokinase (KHK) concentrations in serum, plasma, tissue homogenates, cell lysates. Detection Range: 18.75 mU/ml - 1200 mU/ml.
other info2 :
Sensitivity: The minimum detectable dose of human KHK is typically less than 4.68 mU/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KHK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KHK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KHK is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KHK bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
4557693
ncbi acc num :
NP_000212.1
ncbi gb acc num :
NM_000221.2
uniprot acc num :
P50053
ncbi mol weight :
32,730 Da
ncbi pathways :
Disease Pathway 530764!!Fructose And Mannose Metabolism Pathway 82930!!Fructose And Mannose Metabolism Pathway 291!!Fructose Catabolism Pathway 106218!!Glycogen Storage Diseases Pathway 980468!!Metabolic Pathways 132956!!Metabolism Pathway 477135!!Metabolism Of Carbohydrates Pathway 106196!!Myoclonic Epilepsy Of Lafora Pathway 980469!!Polyol Pathway 198815
ncbi summary :
This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
size :
96 Strip Wells
price :
760 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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