BHLH3 Antibody (N-term) | MyBioSource MBS9208829 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

BHLH3 Antibody (N-term)

catalog :

MBS9208829

quantity :

0.08 mL

price :

155 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-BHLH3 Antibody (N-term) at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

image 2 :

BHLH3 Antibody (N-term) western blot analysis in RD cell line lysates (35ug/lane).This demonstrates the BHE41 antibody detected the BHE41 protein (arrow).

image 3 :

BHLH3 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

product information

catalog number :

MBS9208829

products type :

Antibody

products full name :

BHLH3 Antibody (N-term)

products short name :

[BHLH3]

products name syn :

[Class E basic helix-loop-helix protein 41; bHLHe41; Class B basic helix-loop-helix protein 3; bHLHb3; Differentially expressed in chondrocytes protein 2; hDEC2; Enhancer-of-split and hairy-related protein 1; SHARP-1; BHLHE41; BHLHB3; DEC2; SHARP1]

other names :

[class E basic helix-loop-helix protein 41; Class E basic helix-loop-helix protein 41; class E basic helix-loop-helix protein 41; basic helix-loop-helix family, member e41; Class B basic helix-loop-helix protein 3; bHLHb3; Differentially expressed in chondrocytes protein 2; hDEC2; Enhancer-of-split and hairy-related protein 1; SHARP-1]

products gene name :

[BHLHE41]

other gene names :

[BHLHE41; BHLHE41; DEC2; hDEC2; BHLHB3; SHARP1; BHLHB3; DEC2; SHARP1; bHLHe41; bHLHb3; hDEC2; SHARP-1]

uniprot entry name :

BHE41_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human, Mouse

sequence positions :

[17-46]

sequence length :

482

specificity :

This BHLH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human BHLH3.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)

app notes :

WB~~1:1000. FC~~1:10~50. IF~~1:10~50

image1 heading :

Western Blot

image2 heading :

Western Blot (WB)

image3 heading :

Flow Cytometry (FC/FACS)

image4 heading :

Immunofluorescence (IF)

image4 description :

Fluorescent confocal image of MCF-7 cell stained with BHLH3 Antibody (N-term). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BHLH3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).BHLH3 immunoreactivity is localized to nucleus significantly.

other info1 :

Antigen Source: HUMAN. Function: Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity. Cellular Location: Nucleus {ECO:0000255 PROSITE- ProRule:PRU00380, ECO:0000255 PROSITE-ProRule:PRU00981}. Tissue Location: Highly expressed in skeletal muscle and brain, moderately expressed in pancreas and heart, weakly expressed in placenta, lung, liver and kidney

products categories :

Crown Antibodies; Cancer; Neuroscience; Signal Transduction

products description :

BHLHE41 may be a transcriptional repressor that represses both basal and activated transcription.

products references :

Honma, S., et al. Nature 419(6909):841-844(2002) Garriga-Canut, M., et al. J. Biol. Chem. 276(18):14821-14828(2001) Fujimoto, K., et al. Biochem. Biophys. Res. Commun. 280(1):164-171(2001) Grottke, C., et al. Int. J. Cancer 88(4):535-546(2000)

ncbi gi num :

13540521

ncbi acc num :

NP_110389.1

ncbi gb acc num :

NM_030762.2

uniprot acc num :

Q9C0J9

ncbi pathways :

Taurine And Hypotaurine Metabolism Pathway (79365); Taurine And Hypotaurine Metabolism Pathway (79365); Taurine And Hypotaurine Metabolism Pathway (79365); Taurine And Hypotaurine Metabolism Pathway (79365); Taurine And Hypotaurine Metabolism Pathway (79365)

ncbi summary :

This gene encodes a basic helix-loop-helix protein expressed in various tissues. The encoded protein can interact with ARNTL or compete for E-box binding sites in the promoter of PER1 and repress CLOCK/ARNTL's transactivation of PER1. This gene is believed to be involved in the control of circadian rhythm and cell differentiation. Defects in this gene are associated with the short sleep phenotype. [provided by RefSeq, Feb 2014]

uniprot summary :

BHLHB3: May be a transcriptional repressor that represses both basal and activated transcription. Homodimerize. Chromosomal Location of Human Ortholog: 12p12.1. Cellular Component: nucleus. Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; MRF binding; protein homodimerization activity; protein heterodimerization activity; histone deacetylase binding; bHLH transcription factor binding; transcription corepressor activity. Biological Process: circadian rhythm; transcription from RNA polymerase II promoter; cell proliferation; organ morphogenesis; circadian regulation of gene expression; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; cell differentiation. Disease: Short Sleeper

size1 :

0.08 mL

price1 :

155 USD

size2 :

0.4 mL

price2 :

340

more info or order :

MyBioSource product webpage