catalog number :
MBS920876
products type :
ELISA Kit
products full name :
Human dystroglycan 1 (dystrophin-associated glycoprotein 1) , DAG1 ELISA Kit
products short name :
dystroglycan 1 (dystrophin-associated glycoprotein 1)
products name syn :
Human dystroglycan 1 (dystrophin-associated glycoprotein 1) (DAG1) ELISA kit; 156DAG; A3a; AGRNR; DAG; alpha-dystroglycan; beta-dystroglycan; dystroglycan 1; dystroglycan 1 (dystrophin-associated glycoprotein 1)
other names :
dystroglycan; Dystroglycan; dystroglycan; RAB7, member RAS oncogene family; dystrophin-associated glycoprotein 1; dystroglycan 1 (dystrophin-associated glycoprotein 1); Dystrophin-associated glycoprotein 1Cleaved into the following 2 chains:Alpha-dystroglycan; Alpha-DG; Beta-dystroglycan; Beta-DG
products gene name :
DAG1
other gene names :
DAG1; DAG1; RAB7; Alpha-DG; Beta-DG
uniprot entry name :
DAG1_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of human DAG1. No significant cross-reactivity or interference between human DAG1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 125 pg/ml-8000 pg/ml. Sensitivity: The minimum detectable dose of human DAG1 is typically less than 31.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DAG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DAG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DAG1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DAG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_776587.1
ncbi gb acc num :
NM_174162.1
ncbi mol weight :
97,321 Da
ncbi pathways :
Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117268); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Dilated Cardiomyopathy Pathway (121480); Dilated Cardiomyopathy Pathway (121285); ECM Proteoglycans Pathway (1008850); ECM-receptor Interaction Pathway (84307); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (1008838); Hypertrophic Cardiomyopathy (HCM) Pathway (114091); Hypertrophic Cardiomyopathy (HCM) Pathway (106591)
uniprot summary :
Function: The dystroglycan complex is involved in a number of processes including laminin and basement membrane assembly, sacrolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization
size4 :
10x96-Strip-Wells