CD163 Antibody (N-term) | MyBioSource MBS9208646 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CD163 Antibody (N-term)

catalog :

MBS9208646

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of CD163 antibody (N-term) in K562 cell line lysates (35ug/lane). CD163 (arrow) was detected using the purified Pab.

image 2 :

Formalin-fixed and paraffin-embedded human hepatocarcinoma with CD163 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

product information

catalog number :

MBS9208646

products type :

Antibody

products full name :

CD163 Antibody (N-term)

products short name :

[CD163]

products name syn :

[Scavenger receptor cysteine-rich type 1 protein M130; Hemoglobin scavenger receptor; CD163; Soluble CD163; sCD163; CD163; M130]

other names :

[scavenger receptor cysteine-rich type 1 protein M130 isoform a; Scavenger receptor cysteine-rich type 1 protein M130; scavenger receptor cysteine-rich type 1 protein M130; CD163 molecule; Hemoglobin scavenger receptor; CD_antigen: CD163]

products gene name :

[CD163]

other gene names :

[CD163; CD163; M130; MM130; M130; sCD163]

uniprot entry name :

C163A_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

sequence positions :

[245-274]

sequence length :

1,154

specificity :

This CD163 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 245-274 amino acids from the N-terminal region of human CD163.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

WB, IHC-P, E.

app notes :

WB~~1:1000. IHC-P~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Calculated MW: 125451

other info2 :

Function: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. Cellular location: Soluble CD163: Secreted. Tissue Location: Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.

products categories :

Cardiovascular; Immunology

products description :

CD163 is an acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. The protein may play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. It binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. And it exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. It also induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1.

products references :

Buehler,P.W., Abraham,B. Blood 113 (11), 2578-2586 (2009). Fabriek,B.O., van Bruggen,R. Blood 113 (4), 887-892 (2009). Strauss,M. and Levy,A.P. Mol. Cell. Biochem. 317 (1-2), 131-135 (2008). Groselj-Grenc,M., Ihan,A. Mediators Inflamm. 2008, 202646 (2008)

ncbi gi num :

344179110

ncbi acc num :

NP_004235.4

ncbi gb acc num :

NM_004244.5

uniprot acc num :

Q86VB7

ncbi summary :

The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]

uniprot summary :

CD163: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 12p13.3. Cellular Component: integral to plasma membrane; extracellular region; plasma membrane. Molecular Function: protein binding; scavenger receptor activity. Biological Process: receptor-mediated endocytosis; acute-phase response

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage