TSPAN12 Antibody (C-term) | MyBioSource MBS9208479 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

TSPAN12 Antibody (C-term)

catalog :

MBS9208479

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, flow cytometry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of TSPAN12 Antibody (C-term) in T47D cell line lysates (35ug/lane). TSPAN12 (arrow) was detected using the purified Pab.

image 2 :

Formalin-fixed and paraffin-embedded human brain tissue reacted with TSPAN12 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

image 3 :

TSPAN12 Antibody (C-term) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

product information

catalog number :

MBS9208479

products type :

Antibody

products full name :

TSPAN12 Antibody (C-term)

products short name :

[TSPAN12]

products name syn :

[Tetraspanin-12; Tspan-12; Tetraspan NET-2; Transmembrane 4 superfamily member 12; TSPAN12; NET2; TM4SF12]

other names :

[tetraspanin-12; Tetraspanin-12; tetraspanin-12; tetraspanin 12; Tetraspan NET-2; Transmembrane 4 superfamily member 12]

products gene name :

[TSPAN12]

other gene names :

[TSPAN12; TSPAN12; EVR5; NET2; NET-2; TM4SF12; NET2; TM4SF12; Tspan-12]

uniprot entry name :

TSN12_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human (Predicted Reactivity: Bovine, Mouse, Rat)

sequence positions :

[232-259]

sequence length :

192

specificity :

This TSPAN12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232-259 amino acids from the C-terminal region of human TSPAN12.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

WB, IHC-P, FC,E

app notes :

WB~~1:1000. IHC-P~~1:50~100. FC~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Flow Cytometry (FC/FACS)

other info1 :

Calculated MW: 35383

other info2 :

Function: Regulator of cell surface receptor signal transduction. Plays a central role in retinal vascularization by regulating norrin (NDP) signal transduction. Acts in concert with norrin (NDP) to promote FZD4 multimerization and subsequent activation of FZD4, leading to promote accumulation of beta-catenin (CTNNB1) and stimulateLEF/TCF-mediated transcriptional programs. Suprisingly, it only activate the norrin (NDP)-dependent activation of FZD4, while it does not activate the Wnt-dependent activation of FZD4, suggesting the existence of a Wnt-independent signaling that also promote accumulation the beta-catenin(CTNNB1) (By similarity). Acts as a regulator of membrane proteinases such as ADAM10 and MMP14/MT1-MMP. Activates ADAM10-dependent cleavage activity of amyloid precursor protein (APP). Activates MMP14/MT1-MMP-dependent cleavage activity. Cellular location: Cell membrane; Multi-pass membrane protein

products categories :

Cell Biology; Neuroscience

products description :

TSPAN12 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility.

products references :

Xu,D., et.al., FASEB J. 23 (11), 3674-3681 (2009). Berditchevski,F.et.al., J. Cell. Sci. 114 (PT 23), 4143-4151 (2001)

ncbi gi num :

6912528

ncbi acc num :

NP_036470.1

ncbi gb acc num :

NM_012338.3

uniprot acc num :

O95859

ncbi summary :

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. [provided by RefSeq, Jul 2008]

uniprot summary :

TSPAN12: a multi-pass membrane protein. Member of the transmembrane 4 superfamily, also known as the tetraspanin family. Plays a central role in retinal vascularization by regulating norrin (NDP) signal transduction. Acts in concert with norrin (NDP) to promote FZD4 multimerization and subsequent activation of FZD4, leading to promote accumulation of beta-catenin (CTNNB1) and stimulate LEF/TCF-mediated transcriptional programs. Suprisingly, it only activate the norrin (NDP)-dependent activation of FZD4, while it does not activate the Wnt-dependent activation of FZD4, suggesting the existence of a Wnt-independent signaling that also promote accumulation the beta-catenin (CTNNB1). Acts as a regulator of membrane proteinases such as ADAM10 and MMP14/MT1-MMP. Activates ADAM10-dependent cleavage activity of amyloid precursor protein (APP). Activates MMP14/MT1-MMP-dependent cleavage activity. Defects in TSPAN12 are the cause of vitreoretinopathy exudative type 5 (EVR5). It is a disorder of the retinal vasculature characterized by an abrupt cessation of growth of peripheral capillaries, leading to an avascular peripheral retina. Twp isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, misc. Chromosomal Location of Human Ortholog: 7q31.31. Cellular Component: membrane; integral to plasma membrane; integral to membrane. Molecular Function: Wnt receptor activity. Biological Process: cell surface receptor linked signal transduction; angiogenesis; regulation of angiogenesis. Disease: Exudative Vitreoretinopathy 5

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage