ATG16L Antibody | MyBioSource MBS9208353 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ATG16L Antibody

catalog :

MBS9208353

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Immunofluorescent analysis of U251 cells, using ATG16L Antibody. U251 cells(right) were treated with Chloroquine (50 uM,16h). MBS9208353 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).

image 2 :

APG16L Antibody western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the APG16L antibody detected the APG16L protein (arrow).

image 3 :

Western blot of APG16L (L92) Pab in mouse brain tissue lysate.

product information

catalog number :

MBS9208353

products type :

Antibody

products full name :

ATG16L Antibody

products short name :

[ATG16L]

products name syn :

[Autophagy-related protein 16-1; APG16-like 1; ATG16L1; APG16L]

other names :

[autophagy-related protein 16-1 isoform 4; Autophagy-related protein 16-1; autophagy-related protein 16-1; autophagy related 16-like 1; APG16-like 1]

products gene name :

[ATG16L1]

other gene names :

[ATG16L1; ATG16L1; IBD10; WDR30; APG16L; ATG16A; ATG16L; APG16L]

uniprot entry name :

A16L1_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human, mouse

sequence positions :

[161-190]

specificity :

This ATG16L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-190 amino acids from human ATG16L.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Immunofluorescence (IF), ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

app notes :

IF~~1:25. WB~~1:1000. IHC-P~~1:10~50

image1 heading :

Immunofluorescence (IF)

image2 heading :

Western Blot (WB)

image3 heading :

Western Blot (WB)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with Autophagy APG16L antibody (L176), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

image5 heading :

Western Blot (WB)

image5 description :

Cos7, HEK293, MEF, and Hela cells, left to right, respectively. Data courtesy of Drs. Jiefei Geng and Dan Klionsky, University of Michigan.

other info1 :

Function: Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane (PubMed:<a href="http://www.uni prot.org/citations/24553140" target="_blank">24553140 , PubMed:<a href="http://www.uniprot.org/citations/23376 921" target="_blank">23376921 , PubMed:<a href="http://www.uniprot.org/cita tions/24954904" target="_blank">24954904 , PubMed:<a href="http://www.uniprot.org/citations/27273 576" target="_blank">27273576 , PubMed:<a href="http://www.uniprot.org/cita tions/23392225" target="_blank">23392225 ). Regulates mitochondrial antiviral signaling (MAVS)-dependent type I interferon (IFN-I) production (PubMed:<a href="http://www.uni prot.org/citations/25645662" target="_blank">25645662 ). Negatively regulates NOD1- and NOD2- driven inflammatory cytokine response (PubMed:<a href="http://www.uniprot.org/citations/24238 340" target="_blank">24238340 ). Plays a role in regulating morphology and function of Paneth cell (PubMed:<a href="http://www. uniprot.org/citations/18849966" target="_blank">18849966 ). Cellular Location: Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Note=Recruited to omegasomes membranes by WIPI2 Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme. {ECO:0000250 UniProtKB:Q8C0J2}

products categories :

Neuroscience; Metabolism; Immunology; Cell Biology; Cardiovascular; Cancer; Crown Antibodies; Signal Transduction

products description :

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The APG12-APG5-APG16L complex is esential for the elongation of autophagic isolation membranes. This complex initially associates in uniform distribution with small vesicle membranes. During membrane elongation, the complex partitions, with a great concentration building on the outer side of the isolation membrane. Upon completion of the formation of the autophagosome, the APG12-APG5-APG16L dissociates from the membrane.

products references :

References for protein:. 1.Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005). 2.Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005). 3.Greenberg JT. Dev Cell. 8(6):799-801. (2005). 4.Levine B. Cell. 120(2):159-62. (2005). 5.Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004). References for U251 cell line:. 1. Westermark B.; Pontén J.; Hugosson R. (1973)." Determinants for the establishment of permanent tissue culture lines from human gliomas". Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449]. 2. Pontén, J.,Westermark B. (1978)." Properties of Human Malignant Glioma Cells in Vitro". Medical Biology 56: 184-193.[PMID: 359950]. 3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). "Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent". Neuro Oncol. 12(5): 473-481.[ PMID: 20406898].

ncbi gi num :

298229005

ncbi acc num :

NP_001177195.1

ncbi gb acc num :

NM_001190266.1

uniprot acc num :

Q676U5

ncbi mol weight :

68265

ncbi pathways :

Phenylalanine Metabolism Pathway (55054); Phenylalanine Metabolism Pathway (55054); Phenylalanine Metabolism Pathway (55054)

ncbi summary :

The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2010]

uniprot summary :

ATG16: Plays an essential role in autophagy. Homooligomer. Interacts with ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa). Belongs to the WD repeat ATG16 family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, peripheral; Adaptor/scaffold; Autophagy. Chromosomal Location of Human Ortholog: 2q37.1. Cellular Component: pre-autophagosomal structure membrane; autophagic vacuole; axoneme. Molecular Function: identical protein binding; protein binding. Biological Process: protein transport; protein homooligomerization; autophagic vacuole formation. Disease: Inflammatory Bowel Disease 10

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage