HRI Antibody (N-term) | MyBioSource MBS9208228 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

HRI Antibody (N-term)

catalog :

MBS9208228

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

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image

image 1 :

Western blot analysis of anti-HRI Pab in A375 cell lysate. HRI (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

image 2 :

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

product information

catalog number :

MBS9208228

products type :

Antibody

products full name :

HRI Antibody (N-term)

products short name :

[HRI]

products name syn :

[HRI; KIAA1369]

other names :

[eukaryotic translation initiation factor 2-alpha kinase 1 isoform b; Eukaryotic translation initiation factor 2-alpha kinase 1; eukaryotic translation initiation factor 2-alpha kinase 1; eukaryotic translation initiation factor 2-alpha kinase 1; Heme-controlled repressor; HCR; Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; Heme-regulated inhibitor; Hemin-sensitive initiation factor 2-alpha kinase]

products gene name :

[EIF2AK1]

other gene names :

[EIF2AK1; EIF2AK1; HCR; HRI; HRI; KIAA1369; HCR]

uniprot entry name :

E2AK1_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human

sequence positions :

[1-30]

sequence length :

629

specificity :

This HRI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HRI.

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

concentration :

Vial Concentration: 2

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC-p)

app notes :

WB~~1:1000. IHC-P~~1:50~100

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Cellular Location: Cytoplasm. Tissue Location : Expressed predominantly in erythroid cells. At much lower levels, expressed in hepatocytes (at protein level)

products categories :

Signal Transduction

products description :

Background: The HRI gene is localized to 7p22 where its 3' end slightly overlaps the 3' end of the gene JTV1. The two genes are transcribed from opposite strands. Studies in rat rabbit suggest that the HRI gene product phosphorylates the alpha subunit of eukaryotic initiation factor 2. Its kinase activity is induced by low levels of heme and inhibited by the presence of heme. Function: Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser- 48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions (By similarity).

products references :

Rafie-Kolpin, M., et al., Biochemistry 42(21):6536-6544 (2003). Chen, J.J., et al., Trends Biochem. Sci. 20(3):105-108 (1995).

ncbi gi num :

197245437

ncbi acc num :

NP_001127807.1

ncbi gb acc num :

NM_001134335.1

uniprot acc num :

Q9BQI3

ncbi mol weight :

71106

ncbi pathways :

Citrate Cycle (TCA Cycle) Pathway (27102); Citrate Cycle (TCA Cycle) Pathway (27102); Citrate Cycle (TCA Cycle) Pathway (27102); Citrate Cycle (TCA Cycle) Pathway (27102); Citrate Cycle (TCA Cycle) Pathway (27102); Citrate Cycle (TCA Cycle) Pathway (27102); Citrate Cycle (TCA Cycle) Pathway (27102); Citrate Cycle (TCA Cycle) Pathway (27102); Citrate Cycle (TCA Cycle) Pathway (27102); Citrate Cycle (TCA Cycle) Pathway (27102)

ncbi summary :

The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

uniprot summary :

HRI: a protein kinase of the PEK family. Phosphorylates the alpha subunit of eukaryotic initiation factor 2 in response to various stress conditions, inhibiting protein synthesis at the level of initiation. Its kinase activity is induced by low levels of heme and inhibited by the presence of heme. Binding of nitric oxide (NO) to the heme iron in the N-terminal heme-binding domain activates the kinase activity, while binding of carbon monoxide (CO) suppresses kinase activity. Requires association with a multiprotein complex containing Hsp90, CDC37 and PPP5C for maturation and activation by autophosporylation. Two alternatively spliced isoforms have been described. Protein type: Kinase, protein; Translation; EC 2.7.11.1; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; PEK family. Chromosomal Location of Human Ortholog: 7p22. Cellular Component: cytoplasm. Molecular Function: protein homodimerization activity; heme binding; eukaryotic translation initiation factor 2alpha kinase activity; ATP binding. Biological Process: response to external stimulus; negative regulation of cell proliferation; negative regulation of translational initiation by iron; protoporphyrinogen IX metabolic process; protein amino acid autophosphorylation; response to stress; negative regulation of hemoglobin biosynthetic process

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage