ELISA/assay

Human cortistatin, CORT ELISA Kit

MBS920805

96 Strip Wells

760 USD

ELISA Kit

Human cortistatin, CORT ELISA Kit

cortistatin

Human cortistatin (CORT) ELISA kit; CST-14; CST-17; CST-29; cortistatin-14; cortistatin-17; cortistatin-29; preprocortistatin; cortistatin

cortistatin preproprotein; Cortistatin; cortistatin; cortistatin-14; cortistatin-17; cortistatin-29; preprocortistatin; prepro-cortistatin; cortistatin

CORT

CORT; CORT; CST-14; CST-17; CST-29; UNQ307/PRO350

CORT_HUMAN

Human

105

This assay has high sensitivity and excellent specificity for detection of human CORT. No significant cross-reactivity or interference between human CORT and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of human cortistatin (CORT) concentrations in serum, plasma, tissue homogenates. Detection Range: 0.47 ng/ml-30 ng/ml.

Sensitivity: The minimum detectable dose of human CORT is typically less than 0.12 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CORT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CORT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CORT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CORT bound in the initial step. The color development is stopped and the intensity of the color is measured.

311893369

NP_001293.3

NM_001302.4

O00230

11,532 Da

Cell Cycle Pathway 530733!!Cell Cycle, Mitotic Pathway 105765!!M Phase Pathway 105812!!Mitotic Anaphase Pathway 105818!!Mitotic Metaphase And Anaphase Pathway 730317!!Mitotic Prometaphase Pathway 105815!!Resolution Of Sister Chromatid Cohesion Pathway 730315!!Separation Of Sister Chromatids Pathway 730318

This gene encodes a neuropeptide that is structurally similar to somatostatin. It binds to all known somatostatin receptors, and shares many pharmacological and functional properties with somatostatin, including the depression of neuronal activity. However, it also has many properties distinct from somatostatin, such as induction of slow-wave sleep, apparently by antagonism of the excitatory effects of acetylcholine on the cortex, reduction of locomotor activity, and activation of cation selective currents not responsive to somatostatin. The preproprotein undergoes further processing into multiple mature products. Read-through transcripts exist between this gene and the upstream APITD1 (apoptosis-inducing, TAF9-like domain 1) gene, as represented in GeneID:100526739. [provided by RefSeq, Nov 2010]

Function: Binds to all human somatostatin receptor (SSTR) subtypes. It also inhibits cAMP production induced by forskolin through SSTRs.

96 Strip Wells

760 USD