product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat Platelet-derived growth factor DD, PDGF-DD ELISA Kit
catalog :
MBS920803
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS920803
products type :
ELISA Kit
products full name :
Rat Platelet-derived growth factor DD, PDGF-DD ELISA Kit
products short name :
platelet derived growth factor DD
products name syn :
Rat Platelet-derived growth factor DD (PDGF-DD) ELISA kit; platelet derived growth factor DD
other names :
Platelet-derived growth factor D; Platelet-derived growth factor D; Iris-expressed growth factorCleaved into the following 2 chains:Platelet-derived growth factor D, latent form; PDGFD latent form; Platelet-derived growth factor D, receptor-binding form; PDGFD receptor-binding form
products gene name :
PDGF-DD
other gene names :
PDGFD; IEGF; PDGF-D; PDGFD latent form; PDGFD receptor-binding form
uniprot entry name :
PDGFD_RABIT
reactivity :
Rat
sequence length :
300
specificity :
This assay has high sensitivity and excellent specificity for detection of rat PDGF-DD. No significant cross-reactivity or interference between rat PDGF-DD and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of rat PDGF-DD is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PDGF-DD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDGF-DD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGF-DD is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGF-DD bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
75044560
ncbi acc num :
Q6V9H4.1
uniprot acc num :
Q6V9H4
ncbi mol weight :
34,616 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Plays an important role in wound healing. Induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. Can initiate events that lead to a mesangial proliferative glomerulonephritis, including influx of monocytes and macrophages and production of extracellular matrix
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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