Human Choline/ethanolamine kinase, CHKB ELISA Kit | MyBioSource MBS920777 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Choline/ethanolamine kinase, CHKB ELISA Kit

catalog :

MBS920777

quantity :

48-Strip-Wells

price :

510 USD

product information

catalog number :

MBS920777

products type :

ELISA Kit

products full name :

Human Choline/ethanolamine kinase, CHKB ELISA Kit

products short name :

choline kinase beta

products name syn :

Human Choline/ethanolamine kinase (CHKB) ELISA kit; CHETK; CHKL; CKEKB; EKB; choline kinase-like; choline/ethanolamine kinase; choline kinase beta

other names :

choline/ethanolamine kinase; Choline/ethanolamine kinase; choline/ethanolamine kinase; ethanolamine kinase beta; choline kinase-like protein; choline kinase beta; Choline kinase beta (EC:2.7.1.32); CK; CKB; Choline kinase-like protein; Ethanolamine kinase (EC:2.7.1.82); EK; Ethanolamine kinase beta; EKB; choline/ethanolamine kinase beta; CKEKB

products gene name :

CHKB

other gene names :

CHKB; CHKB; CK; EK; CKB; EKB; CHKL; CHETK; CKEKB; MDCMC; CHETK; CHKL; CK; CKB; EK; EKB; CKEKB

uniprot entry name :

CHKB_HUMAN

reactivity :

Human

sequence length :

395

specificity :

This assay has high sensitivity and excellent specificity for detection of human CHKB. No significant cross-reactivity or interference between human CHKB and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 23.44 pg/ml-1500 pg/ml. Sensitivity: The minimum detectable dose of human CHKB is typically less than 5.86 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CHKB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHKB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHKB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHKB bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

6978649

ncbi acc num :

NP_005189.2

ncbi gb acc num :

NM_005198.4

uniprot acc num :

Q9Y259

ncbi mol weight :

45,271 Da

ncbi pathways :

Fatty Acid Beta Oxidation Pathway (198865); Glycerophospholipid Biosynthesis Pathway (645313); Glycerophospholipid Metabolism Pathway (82989); Glycerophospholipid Metabolism Pathway (364); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Phosphatidylcholine (PC) Biosynthesis, Choline = PC Pathway (413385); Phosphatidylcholine (PC) Biosynthesis, Choline = PC Pathway (468283); Phosphatidylethanolamine (PE) Biosynthesis, Ethanolamine = PE Pathway (413386)

ncbi summary :

Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus. [provided by RefSeq, Jun 2009]

uniprot summary :

CHKB: Has a key role in phospholipid biosynthesis. Catalyzes the first step in phosphatidylethanolamine biosynthesis. Phosphorylates ethanolamine, and can also act on choline (in vitro). Has higher activity with ethanolamine. May not significantly contribute to in vivo phosphatidylcholine biosynthesis. Belongs to the choline/ethanolamine kinase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 2.7.1.82; Lipid Metabolism - glycerophospholipid; Kinase, other; EC 2.7.1.32. Chromosomal Location of Human Ortholog: 22q13.33. Cellular Component: cytosol. Molecular Function: choline kinase activity; ethanolamine kinase activity; ATP binding. Biological Process: CDP-choline pathway; phospholipid metabolic process; phosphatidylethanolamine biosynthetic process; glycerophospholipid biosynthetic process; phosphatidylcholine biosynthetic process; phosphorylation. Disease: Muscular Dystrophy, Congenital, Megaconial Type

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800