PRODH Antibody (Center) | MyBioSource MBS9207635 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

PRODH Antibody (Center)

catalog :

MBS9207635

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, flow cytometry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Overlay histogram showing A549 cells stainedwith MBS9207635 (green line). The cells were fixedwith 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.The cells were then icubated in 2% bovine serum albumin to block non-specificprotein-protein interactions followed by the antibody (MBS9207635, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used wasGoat-Anti-Rabbit IgG, DyLight® 488Conjugated Highly Cross-Adsorbed(OH191631)at 1/200 dilution for 40 min at 37ºC. Isotypecontrol antibody (blue line) was rabbit IgG(1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

image 2 :

MBS9207635_IHC staining PRODH in H. skeletal musclesections by Immunohistochemistry (IHC-P paraformaldehyde-fixed, paraffin-embeddedsections). Tissue was fixed with formaldehydeand blocked with 3% BSA for 0. 5 hour at roomtemperature; antigen retrieval was by heatmediation with a citrate buffer (pH6). Sampleswere incubated with primary antibody (1/25)for 1 hours at 37°C. A undiluted biotinylatedgoat polyvalent antibody was used as the secondary antibody.

image 3 :

Anti-PRODH Antibody (Center) at 1: 2000 dilution + MDA-MB-468 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidaseconjugated at 1/10000 dilution. Predicted bandsize : 68 kDa Blocking/Dilution buffer: 5%NFDM/TBST.

product information

catalog number :

MBS9207635

products type :

Antibody

products full name :

PRODH Antibody (Center)

products short name :

[PRODH]

products name syn :

[Proline dehydrogenase 1; mitochondrial; Proline oxidase; Prodh; Pro1]

other names :

[proline dehydrogenase 1, mitochondrial; Proline dehydrogenase 1, mitochondrial; proline dehydrogenase 1, mitochondrial; proline dehydrogenase; Proline oxidase]

products gene name :

[Prodh]

other gene names :

[Prodh; Prodh; Pro1; Pro-1; Ym24d07; Pro1]

uniprot entry name :

PROD_MOUSE

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human, mouse

sequence positions :

[130-155]

sequence length :

599

specificity :

This PRODH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130-155 amino acids from the Central region of human PRODH.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Flow Cytometry (FC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB), ELISA (EIA)

app notes :

FC~~1:25. IHC-P~~1:50~100. WB~~1:1000

image1 heading :

Flow Cytometry FC

image2 heading :

Immunohistochemistry IHC

image3 heading :

Western Blot WB

image4 heading :

Testing Data

image4 description :

Anti-PRODH Antibody (Center) at 1:2000dilution + human brain lysate Lysates/proteinsat 20 µg per lane. Secondary Goat Anti-RabbitIgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDaBlocking/Dilution buffer: 5% NFDM/TBST.

image5 heading :

Western Blot WB

image5 description :

All lanes : Anti-PRODH Antibody (Center) at1:1000 dilution Lane 1: MDA-MB-468 whole celllysates Lane 2: A549 whole cell lysatesLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted bandsize : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

other info1 :

Function: Converts proline to delta-1-pyrroline-5-carboxylate. Cellular Location: Mitochondrion matrix. Tissue Location: Expressed in liver, kidney, heart and to a lesser extent in brain, lung and muscle.

products categories :

Cancer; Cell Biology; Signal Transduction

products description :

PRODH is a mitochondrial proline dehydrogenase that catalyzes the first step in proline degradation. It converts proline to delta-1-pyrroline-5-carboxylate.

products references :

Polyak,K., et.al., Nature 389 (6648), 300-305 (1997) Gogos,J.A., et.al., Nat. Genet. 21 (4), 434-439 (1999)

ncbi gi num :

118601004

ncbi acc num :

NP_035302.2

ncbi gb acc num :

NM_011172.2

uniprot acc num :

Q9WU79

uniprot summary :

PRODH: Converts proline to delta-1-pyrroline-5-carboxylate. Defects in PRODH are the cause of hyperprolinemia type 1 (HP-1). HP-1 is a disorder characterized by elevated serum proline levels. May be involved in the psychiatric and behavioral phenotypes associated with the 22q11 velocardiofacial and DiGeorge syndrome. Defects in PRODH are associated with susceptibility to schizophrenia type 4 (SCZD4). A complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder. Belongs to the proline oxidase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Oxidoreductase; Mitochondrial; EC 1.5.5.2; Amino Acid Metabolism - arginine and proline. Cellular Component: mitochondrion; mitochondrial inner membrane. Molecular Function: amino acid binding; proline dehydrogenase activity; oxidoreductase activity. Biological Process: proline catabolic process; proline metabolic process

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage