Phospho-ARHGAP35 (Y1105) antibody | MyBioSource MBS9207595 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Phospho-ARHGAP35 (Y1105) antibody

catalog :

MBS9207595

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of lysates from A431 cell line, untreated or treated with EGF, 100ng/ml, using Phospho-HUMAN-ARHGAP35(Y1105) (upper) or Beta-actin (lower).

product information

catalog number :

MBS9207595

products type :

Antibody

products full name :

Phospho-ARHGAP35 (Y1105) antibody

products short name :

[Phospho-ARHGAP35 (Y1105)]

products name syn :

[Rho GTPase-activating protein 35; Glucocorticoid receptor DNA-binding factor 1; Glucocorticoid receptor repression factor 1; GRF-1; Rho GAP p190A; p190-A; ARHGAP35; GRF1; GRLF1; KIAA1722]

other names :

[rho GTPase-activating protein 35; Rho GTPase-activating protein 35; rho GTPase-activating protein 35; Rho GTPase activating protein 35; Glucocorticoid receptor DNA-binding factor 1; Glucocorticoid receptor repression factor 1; GRF-1; Rho GAP p190A; p190-A]

products gene name :

[ARHGAP35]

other gene names :

[ARHGAP35; ARHGAP35; GRF-1; GRLF1; P190A; P190-A; p190RhoGAP; p190ARhoGAP; GRF1; GRLF1; KIAA1722; GRF-1; p190-A]

uniprot entry name :

RHG35_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human (Predicted Reactivity: Mouse, Rat)

sequence length :

1,499

specificity :

This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1090-1135 amino acids from human.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

concentration :

Vial Concentration: 0.5

storage stability :

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA)

app notes :

WB~~1:500

image1 heading :

Western Blot (WB)

other info1 :

Clone Names: RB46920

other info2 :

Antigen Source: HUMAN. Function: Rho GTPase-activating protein (GAP), binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity (PubMed: 19673492 ). This binding is inhibited by phosphorylation by PRKCA (PubMed: 19673492 ). Involved in cell differentiation as well as cell adhesion and migration, plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis (By similarity). Transduces signals from p21-ras to the nucleus, acting via the ras GTPase- activating protein (GAP) (By similarity). Transduces SRC-dependent signal from cell-surface adhesion mo lecules, such as laminin, to promote neurite outgrowth. Regulates axon outgrowth, guidance and fasciculation (By similarity). Modulates Rho GTPase-dependent F- actin polymerization, organization and assembly, is involved in polarized cell migration and in the positive regulation of ciliogenesis and cilia elongation (By similarity). During mammary gland development, is required in both the epithelial and stromal compartments for ductal outgrowth (By similarity). Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'; this function is however unclear and would need additional experimental evidences (PubMed: 1894621 ). Cellular Location: Cytoplasm {ECO:0000250 UniProtKB:Q91YM2}. Cytoplasm, cytoskeleton, cilium basal body {ECO:0000250 UniProtKB:Q91YM2}. Cell membrane {ECO:0000250 UniProtKB:Q91YM2}. Nucleus. Note=In response to integrins and SDC4 and upon phosphorylation by PKC, relocalizes from the cytoplasm to regions of plasma membrane ruffling where it colocalizes with polymerized actin {ECO:0000250 UniProtKB:Q91YM2}. Tissue Location: Detected in neutrophils (at protein level)

products categories :

Phospho Antibodies

products description :

Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'- GAGAAAAGAAACTGGAGAAACTC-3'. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). May also act as a tumor suppressor.

products references :

Nagase T.,et al.DNA Res. 7:347-355(2000). Nakajima D.,et al.DNA Res. 9:99-106(2002). Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. Tikoo A.,et al.Gene 257:23-31(2000). LeClerc S.,et al.J. Biol. Chem. 266:17333-17340(1991).

ncbi gi num :

150417981

ncbi acc num :

NP_004482.4

ncbi gb acc num :

NM_004491.4

uniprot acc num :

Q9NRY4

ncbi mol weight :

170514

ncbi summary :

The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008]

uniprot summary :

GRF-1: the human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTP-ase activating protein (GAP). May also act as a tumor suppressor. Two splice variant isoforms have been described. Protein type: GAPs, Rac/Rho; Tumor suppressor; Motility/polarity/chemotaxis; Transcription factor; GAPs. Chromosomal Location of Human Ortholog: 19q13.3. Cellular Component: cytosol; nucleus; actin cytoskeleton. Molecular Function: GTP binding; DNA binding; transcription corepressor activity. Biological Process: regulation of cell shape; axon guidance; regulation of small GTPase mediated signal transduction; camera-type eye development; negative regulation of Rho protein signal transduction; transcription, DNA-dependent; small GTPase mediated signal transduction; neural tube closure; forebrain development; negative regulation of vascular permeability; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage