LPL Antibody (Center) | MyBioSource MBS9207576 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

LPL Antibody (Center)

catalog :

MBS9207576

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Overlay histogram showing Hela cells stained with MBS9207576 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MBS9207576, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/400 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was Rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

image 2 :

All lanes : Anti-LPL Antibody (Center) at 1:1000-1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 53 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

image 3 :

LPL Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the LPL antibody detected the LPL protein (arrow).

product information

catalog number :

MBS9207576

products type :

Antibody

products full name :

LPL Antibody (Center)

products short name :

[LPL]

products name syn :

[Lipoprotein lipase; LPL; LPL; LIPD]

other names :

[lipoprotein lipase; Lipoprotein lipase; lipoprotein lipase; lipoprotein lipase]

products gene name :

[LPL]

other gene names :

[LPL; LPL; LIPD; HDLCQ11; LIPD; LPL]

uniprot entry name :

LIPL_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human (Predicted Reactivity: Bovine, Mouse, Pig, Rat)

sequence positions :

[300-327]

sequence length :

475

specificity :

This LPL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300-327 amino acids from the Central region of human LPL.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

app notes :

FC~~1:25. WB~~1:1000. IHC-P~~1:10~50

image1 heading :

Flow Cytometry

image2 heading :

Western Blot

image3 heading :

Western Blot (WB)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

LPL Antibody (Center) (MBS9207576)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LPL Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

other info1 :

Antigen Type: Synthetic Peptide

other info2 :

Antigen Source: HUMAN. Function: The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium (By similarity). Cellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Secreted. Note=Locates to the plasma membrane of microvilli of hepatocytes with triacyl- glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles (By similarity).

products categories :

Cancer; Cardiovascular; Metabolism; Signal Transduction

products description :

LPL encodes lipoprotein lipase, which is expressed in. heart, muscle, and adipose tissue. LPL functions as a homodimer,. and has the dual functions of triglyceride hydrolase and. ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I. hyperlipoproteinemia, while less extreme mutations in LPL are. linked to many disorders of lipoprotein metabolism. [provided by. RefSeq].

products references :

Hu, M., et al. Pharmacogenet. Genomics 20(10):634-637(2010). Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :. Johansen, C.T., et al. Nat. Genet. 42(8):684-687(2010). Zabaneh, D., et al. PLoS ONE 5 (8) (2010) :. Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :

ncbi gi num :

4557727

ncbi acc num :

NP_000228.1

ncbi gb acc num :

NM_000237.2

uniprot acc num :

P06858

ncbi summary :

LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism. [provided by RefSeq, Jul 2008]

uniprot summary :

LPL: The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium. Defects in LPL are the cause of lipoprotein lipase deficiency (LPL deficiency); also known as familial chylomicronemia or hyperlipoproteinemia type I. LPL deficiency chylomicronemia is a recessive disorder usually manifesting in childhood. On a normal diet, patients often present with abdominal pain, hepatosplenomegaly, lipemia retinalis, eruptive xanthomata, and massive hypertriglyceridemia, sometimes complicated with acute pancreatitis. Belongs to the AB hydrolase superfamily. Lipase family. Protein type: EC 3.1.1.34; Lipid Metabolism - glycerolipid; Membrane protein, GPI anchor; Phospholipase. Chromosomal Location of Human Ortholog: 8p22. Cellular Component: extracellular matrix; extracellular space; chylomicron; cell surface; plasma membrane; extracellular region. Molecular Function: heparin binding; triacylglycerol lipase activity; lipoprotein lipase activity; protein binding; apolipoprotein binding; phospholipase activity; triglyceride binding; receptor binding. Biological Process: response to drug; phototransduction, visible light; triacylglycerol metabolic process; phospholipid metabolic process; triacylglycerol catabolic process; triacylglycerol biosynthetic process; lipoprotein metabolic process; response to cold; retinoid metabolic process; fatty acid biosynthetic process. Disease: Hyperlipoproteinemia, Type I; Hyperlipidemia, Familial Combined

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage