SPARC Antibody (C-term) | MyBioSource MBS9207505 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

SPARC Antibody (C-term)

catalog :

MBS9207505

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, chicken

application :

western blot, ELISA, immunohistochemistry, flow cytometry, FACS, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of SPARC Antibody (C-term) in Y79 cell line lysates (35ug/lane). SPARC (arrow) was detected using the purified Pab.

image 2 :

Western blot analysis of SPARC (arrow) using rabbit polyclonal SPARC Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPARC gene.

image 3 :

Formalin-fixed and paraffin-embedded human skin tissue reacted with SPARC Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

product information

catalog number :

MBS9207505

products type :

Antibody

products full name :

SPARC Antibody (C-term)

products short name :

[SPARC]

products name syn :

[SPARC; Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine; SPARC; ON]

other names :

[SPARC isoform 1; SPARC; SPARC; secreted protein, acidic, cysteine-rich (osteonectin); Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine]

products gene name :

[SPARC]

other gene names :

[SPARC; SPARC; ON; BM-40; ON; BM-40; ON]

uniprot entry name :

SPRC_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human (Predicted Reactivity: Bovine, Chicken, Mouse, Pig, Rabbit, Rat, Xenopus)

sequence positions :

[224-251]

sequence length :

303

specificity :

This SPARC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-251 amino acids from the C-terminal region of human SPARC.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC-P), Flow Cytometry (FC/FACS)

app notes :

WB~~1:1000. IHC-P~~1:10~50. FC~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Flow Cytometry (FC/FACS)

image4 description :

Flow cytometric analysis of widr cells using SPARC Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

other info1 :

Function: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. Cellular Location: Secreted, extracelluar matrix, basement membrane. Note= In or around the basement membrane.

products categories :

Cancer; Cardiovascular; Cell Biology; Immunology

products description :

Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM).

products references :

Said,N., Oncogene 28 (39), 3487-3498 (2009). Maloney,S.C., Anticancer Res. 29 (8), 3059-3064 (2009)

ncbi gi num :

4507171

ncbi acc num :

NP_003109.1

ncbi gb acc num :

NM_003118.3

uniprot acc num :

P09486

ncbi mol weight :

34632

ncbi pathways :

Taurine And Hypotaurine Metabolism Pathway (6678); Taurine And Hypotaurine Metabolism Pathway (6678); Taurine And Hypotaurine Metabolism Pathway (6678); Taurine And Hypotaurine Metabolism Pathway (6678); Taurine And Hypotaurine Metabolism Pathway (6678); Taurine And Hypotaurine Metabolism Pathway (6678); Taurine And Hypotaurine Metabolism Pathway (6678); Taurine And Hypotaurine Metabolism Pathway (6678); Taurine And Hypotaurine Metabolism Pathway (6678); Taurine And Hypotaurine Metabolism Pathway (6678)

ncbi summary :

This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015]

uniprot summary :

SPARC: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. Belongs to the SPARC family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 5q31.3-q32. Cellular Component: extracellular space; platelet alpha granule membrane; nuclear matrix; cell surface; mitochondrion; cytoplasm; extracellular region; plasma membrane; basement membrane. Molecular Function: collagen binding; protein binding; extracellular matrix binding; calcium ion binding. Biological Process: response to gravity; response to peptide hormone stimulus; platelet activation; receptor-mediated endocytosis; extracellular matrix organization and biogenesis; ossification; response to cAMP; heart development; response to glucocorticoid stimulus; response to lipopolysaccharide; signal transduction; response to L-ascorbic acid; negative regulation of angiogenesis; response to cadmium ion; platelet degranulation; response to ethanol; response to cytokine stimulus; response to lead ion; negative regulation of endothelial cell proliferation; regulation of cell morphogenesis; blood coagulation; response to calcium ion; inner ear development; lung development. Disease: Osteogenesis Imperfecta, Type Xvii

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage