ADCY10 Antibody (Center) | MyBioSource MBS9207388 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ADCY10 Antibody (Center)

catalog :

MBS9207388

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-ADCY10 Antibody (Center) at 1:1000 dilution + K562 whole cell lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 187 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

image 2 :

ADCY10 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

product information

catalog number :

MBS9207388

products type :

Antibody

products full name :

ADCY10 Antibody (Center)

products short name :

[ADCY10]

products name syn :

[Adenylate cyclase type 10; AH-related protein; Adenylate cyclase homolog; Germ cell soluble adenylyl cyclase; hsAC; sAC; Testicular soluble adenylyl cyclase; ADCY10; SAC]

other names :

[adenylate cyclase type 10 isoform 2; Adenylate cyclase type 10; adenylate cyclase type 10; adenylate cyclase 10 (soluble); AH-related protein; Adenylate cyclase homolog; Germ cell soluble adenylyl cyclase; hsAC; sAC; Testicular soluble adenylyl cyclase]

products gene name :

[ADCY10]

other gene names :

[ADCY10; ADCY10; SAC; HCA2; SACI; Sacy; hsAC; SAC; hsAC; sAC]

uniprot entry name :

ADCYA_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human

sequence positions :

[557-583]

sequence length :

1,457

specificity :

This ADCY10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 557-583 amino acids from the Central region of human ADCY10.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

concentration :

Vial Concentration: 0.42

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)

app notes :

WB: 1:1000. FC: 1:10-50

image1 heading :

Western Blot (WB)

image2 heading :

Flow Cytometry (FC/FACS)

other info1 :

Antigen Type: Synthetic Peptide. Clone Names: RB22128

other info2 :

Antigen Source: HUMAN. Function: Catalyzes the formation of the signaling molecule cAMP (PubMed: 12609998 , PubMed: 15659711 ,PubMed: 24616449 , PubMed: 25040695 ,PubMed: 24567411 ). May function as sensor that mediates responses to changes in cellular bicarbonate and CO(2) levels (PubMed: 15659711 , PubMed: 17591988 ). Has a critical role in mammalian spermatogenesis by producing the cAMP which regulates cAMP-responsive nuclear factors indispensable for sperm maturation in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization (By similarity). Involved inciliary beat regulation (PubMed: 17591988 ). Cellular Location: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region Nucleus. Cell projection, cilium. Cytoplasm. Mitochondrion. Note=Distributed to subcellular compartments containing cAMP targets. Found as a plasma membraneassociated protein, protein concentrated in the perinuclear region and protein colocalized with actin or tubulin. Tissue Location: Detected in airway epithelial cells and testis (at protein level) (PubMed:17591988). Weakly expressed in multiple tissues. Expressed in brain, heart, kidney, liver, lung, pancreas, peripheral blood leukocytes, placenta, skeletal muscle, stomach, thymus, airway epithelial cells, duodenum, jejunum and ileum. Very low level of expression in bone.

products categories :

Cancer; Signal Transduction

ncbi gi num :

268370055

ncbi acc num :

NP_001161221.1

ncbi gb acc num :

NM_001167749.2

uniprot acc num :

Q96PN6

ncbi mol weight :

187149

ncbi pathways :

Valine, Leucine And Isoleucine Biosynthesis Pathway (55811); Valine, Leucine And Isoleucine Biosynthesis Pathway (55811); Valine, Leucine And Isoleucine Biosynthesis Pathway (55811); Valine, Leucine And Isoleucine Biosynthesis Pathway (55811); Valine, Leucine And Isoleucine Biosynthesis Pathway (55811); Valine, Leucine And Isoleucine Biosynthesis Pathway (55811); Valine, Leucine And Isoleucine Biosynthesis Pathway (55811); Valine, Leucine And Isoleucine Biosynthesis Pathway (55811); Valine, Leucine And Isoleucine Biosynthesis Pathway (55811); Valine, Leucine And Isoleucine Biosynthesis Pathway (55811)

ncbi summary :

The protein encoded by this gene belongs to a distinct class of adenylyl cyclases that is soluble and insensitive to G protein or forskolin regulation. Activity of this protein is regulated by bicarbonate. Variation at this gene has been observed in patients with absorptive hypercalciuria. Alternatively spliced transcript variants encoding different isoforms have been observed. There is a pseudogene of this gene on chromosome 6. [provided by RefSeq, Jul 2014]

uniprot summary :

SAC: Soluble adenylyl cyclase that has a critical role in mammalian spermatogenesis. Produces the cAMP which mediates in part the cAMP-responsive nuclear factors indispensable for maturation of sperm in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization. May be the bicarbonate sensor. Involved in ciliary beat regulation. Genetic variations in ADCY10 are a cause of susceptibility to hypercalciuria absorptive type 2 (HCA2). Absorptive hypercalciuria is a common type of hypercalciuria, a condition characterized by excessive urinary calcium excretion. Absorptive hypercalciuria is due to gastrointestinal hyperabsorption of calcium and is a frequent cause of calcium oxalate nephrolithiasis. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: EC 4.6.1.1; Lyase; Adenylyl cyclase; Nucleotide Metabolism - purine. Chromosomal Location of Human Ortholog: 1q24. Cellular Component: growth cone; cytoskeleton; cell soma; axon; apical part of cell; perinuclear region of cytoplasm; cytoplasm; dendrite; plasma membrane; cytosol. Molecular Function: manganese ion binding; magnesium ion binding; ATP binding; ATPase binding; adenylate cyclase activity. Biological Process: positive regulation of apoptosis; cAMP biosynthetic process; spermatogenesis. Disease: Hypercalciuria, Absorptive, 2

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage