PAK4 Antibody (N-term) | MyBioSource MBS9207169 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

PAK4 Antibody (N-term)

catalog :

MBS9207169

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PAK4 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

image 2 :

The anti-PAK4 Pab is used in Western blot to detect PAK4 in mouse small intestine tissue lysate.

product information

catalog number :

MBS9207169

products type :

Antibody

products full name :

PAK4 Antibody (N-term)

products short name :

[PAK4]

products name syn :

[Serine/threonine-protein kinase PAK 4; p21-activated kinase 4; PAK-4; PAK4; KIAA1142]

other names :

[serine/threonine-protein kinase PAK 4 isoform 1; Serine/threonine-protein kinase PAK 4; serine/threonine-protein kinase PAK 4; p21 protein (Cdc42/Rac)-activated kinase 4; p21-activated kinase 4; PAK-4]

products gene name :

[PAK4]

other gene names :

[PAK4; PAK4; KIAA1142; PAK-4]

uniprot entry name :

PAK4_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human, mouse

sequence positions :

[156-187]

sequence length :

501

specificity :

This PAK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-187 amino acids from the N-terminal region of human PAK4.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

app notes :

IHC~~1:10~50. WB~~1:1000

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

other info1 :

Calculated MW: 64072

other info2 :

Function: Serine/threonine protein kinase that plays arole in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN. Cellular Location: Cytoplasm. Note=Seems to shuttle betweencytoplasmic compartments depending on the activating effector. For example, can be found on the cell periphery after activation of growth-factor or integrin-mediated signaling pathways. Tissue Location: Highest expression in prostate, testis and colon.

products categories :

Cancer; Cell Biology; Signal Transduction

products description :

PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3, PAK4, PAK5, and PAK6. PAK proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton.

products references :

Lu, Y., et al., J. Biol. Chem. 278(12):10374-10380 (2003). Bagrodia, S., et al., Trends Cell Biol. 9(9):350-355 (1999). Abo, A., et al., EMBO J. 17(22):6527-6540 (1998).

ncbi gi num :

62422554

ncbi acc num :

NP_001014831.1

ncbi gb acc num :

NM_001014831.2

uniprot acc num :

O96013

ncbi pathways :

Thiamine Metabolism Pathway (10298); Thiamine Metabolism Pathway (10298); Thiamine Metabolism Pathway (10298); Thiamine Metabolism Pathway (10298); Thiamine Metabolism Pathway (10298); Thiamine Metabolism Pathway (10298); Thiamine Metabolism Pathway (10298); Thiamine Metabolism Pathway (10298); Thiamine Metabolism Pathway (10298); Thiamine Metabolism Pathway (10298)

ncbi summary :

PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

PAK4: a protein kinase of the STE20 family. A mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Interacts specifically with the GTP-bound form of Cdc42 and weakly activates the JNK family of MAP kinases. Overexpressed in several cancers. Required for ras-dependent anchorage-independent growth of tumor cell lines. Four alternatively spliced human isoforms have been reported. Protein type: Protein kinase, STE; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; PAKB subfamily. Chromosomal Location of Human Ortholog: 19q13.2. Cellular Component: Golgi apparatus; focal adhesion. Molecular Function: protein binding; ATP binding; receptor signaling protein serine/threonine kinase activity; protein kinase activity. Biological Process: cell proliferation; cell migration; apoptosis; cytoskeleton organization and biogenesis; mitotic cell cycle; signal transduction; cell growth; cell motility; protein amino acid phosphorylation

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage