AMH Antibody (Center) | MyBioSource MBS9207040 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

AMH Antibody (Center)

catalog :

MBS9207040

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, flow cytometry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

MBS9207040 staining AMH in human cerebellum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

image 2 :

Overlay histogram showing SH-SY5Y cells stained with AP9940c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MBS9207040, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1?g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

image 3 :

All lanes : Anti-AMH Antibody (Center) at 1:2000 dilution . Lane 1: 293 whole cell lysate . Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

product information

catalog number :

MBS9207040

products type :

Antibody

products full name :

AMH Antibody (Center)

products short name :

[AMH]

products name syn :

[Muellerian-inhibiting factor, Anti-Muellerian hormone, AMH, Muellerian-inhibiting substance, MIS, AMH, MIF]

other names :

[muellerian-inhibiting factor; Muellerian-inhibiting factor; muellerian-inhibiting factor; anti-Mullerian hormone; Anti-Muellerian hormone; AMH; Muellerian-inhibiting substance; MIS]

products gene name :

[AMH]

other gene names :

[AMH; AMH; MIF; MIS; MIF; AMH; MIS]

uniprot entry name :

MIS_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human, mouse

sequence positions :

[424-451]

sequence length :

560

specificity :

This AMH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 424-451 amino acids from the Central region of human AMH.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

WB, IHC-P, FC,E

app notes :

IHC-P~~1:25. FC~~1:25. WB~~1:2000

image1 heading :

Testing Data(TD)

image2 heading :

Testing Data (TD)

image3 heading :

Testing Data (TD)

other info2 :

Function: This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin. Cellular Location: Secreted.

products categories :

Immunology

products description :

Anti mullerian hormone (AMH) is a member of the TGF beta superfamily. It is secreted as a homodimeric 140kD disulphide linked precursor that is cleaved to release the mature 30kD homodimer. Originally classified as a foetal testicular hormone that inhibits Mullerian duct development, AMH is expressed post natally by immature Sertoli cells, and to a lesser degree by granulosa cells. AMH plays a role in testicular differentiation and in the regulation of ovarian follicle growth.

ncbi gi num :

157266298

ncbi acc num :

NP_000470.2

ncbi gb acc num :

NM_000479.3

uniprot acc num :

P03971

ncbi mol weight :

59195

ncbi pathways :

, Pathway (268); , Pathway (268); , Pathway (268); , Pathway (268); , Pathway (268); , Pathway (268); , Pathway (268); , Pathway (268); , Pathway (268); , Pathway (268)

ncbi summary :

Anti-Mullerian hormone is a member of the transforming growth factor-beta gene family which mediates male sexual differentiation. Anti-Mullerian hormone causes the regression of Mullerian ducts which would otherwise differentiate into the uterus and fallopian tubes. Some mutations in the anti-Mullerian hormone result in persistent Mullerian duct syndrome. [provided by RefSeq, Jul 2008]

uniprot summary :

AMH: This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin. Defects in AMH are the cause of persistent Muellerian duct syndrome type 1 (PMDS1). PMDS1 is a form of male pseudohermaphroditism characterized by a failure of Muellerian duct regression in otherwise normal males. Belongs to the TGF-beta family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: extracellular space; cytoplasm; extracellular region. Molecular Function: growth factor activity; hormone activity; transforming growth factor beta receptor binding; receptor binding. Biological Process: sex determination; response to drug; Mullerian duct regression; cell-cell signaling; preantral ovarian follicle growth; gonadal mesoderm development; sex differentiation; response to organic cyclic substance; urogenital system development; aging; activation of NF-kappaB transcription factor. Disease: Persistent Mullerian Duct Syndrome, Types I And Ii

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage