product summary
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company name :
MyBioSource
product type :
antibody
product name :
C19orf21 Antibody (N-term)
catalog :
MBS9206968
quantity :
0.08 mL
price :
165 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, flow cytometry, FACS, enzyme immunoassay
more info or order :
image
image 1 :
Western blot analysis of C19orf21 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). C19orf21 (arrow) was detected using the purified Pab.
image 2 :
C19orf21 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the C19orf21 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
image 3 :
C19orf21 Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
product information
catalog number :
MBS9206968
products type :
Antibody
products full name :
C19orf21 Antibody (N-term)
products short name :
[C19orf21]
products name syn :
[Mitotic interactor and substrate of PLK1; Mitotic spindle positioning protein; MISP; C19orf21]
other names :
[mitotic interactor and substrate of PLK1; Mitotic interactor and substrate of PLK1; mitotic interactor and substrate of PLK1; mitotic spindle positioning; Mitotic spindle positioning protein]
products gene name :
[MISP]
other gene names :
[MISP; MISP; C19orf21; C19orf21]
uniprot entry name :
MISP_HUMAN
clonality :
Polyclonal
isotype :
Rabbit Ig
host :
Rabbit
reactivity :
Human
sequence positions :
[63-91]
sequence length :
679
specificity :
This C19orf21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-91 amino acids from the N-terminal region of human C19orf21.
purity :
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
form :
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
concentration :
Vial Concentration: 0.5
storage stability :
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
tested application :
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
app notes :
WB~~1:1000. IHC-P~~1:50~100. FC~~1:10~50
image1 heading :
Western Blot (WB)
image2 heading :
Immunohistochemistry (IHC)
image3 heading :
Flow Cytometry (FC/FACS)
other info1 :
Antigen Type: Synthetic Peptide. Cellular Location: Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Note=Predominantly localizes to cortical actin structures during interphase and mitosis Present in retraction fibers, which are formed at former adhesion sites during mitosis, and at spicular membrane protrusions in re- attaching cytokinetic cells. Partially colocalizes with cytoplasmic F-actin. Not detected at microtubules at interphase, nor at spindle during mitosis
other info2 :
Antigen Source: HUMAN
products categories :
Cell Biology
products description :
The exact function of C19orf21 remains unknown.
products references :
Olsen, J.V., et al. Cell 127(3):635-648(2006). Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006). Kim, J.E., et al. J. Proteome Res. 4(4):1339-1346(2005)
ncbi gi num :
27735067
ncbi acc num :
NP_775752.1
ncbi gb acc num :
NM_173481.2
uniprot acc num :
Q8IVT2
ncbi mol weight :
75357
uniprot summary :
MISP: Plays a role in mitotic spindle orientation and mitotic progression. Regulates the distribution of dynactin at the cell cortex in a PLK1-dependent manner, thus stabilizing cortical and astral microtubule attachments required for proper mitotic spindle positioning. May link microtubules to the actin cytospkeleton and focal adhesions. May be required for directed cell migration and centrosome orientation. May also be necessary for proper stacking of the Golgi apparatus. Associates with F-actin. Interacts with DCTN1; this interaction regulates DCTN1 distribution at the cell cortex. Interacts with PTK2/FAK and MAPRE1. Protein type: Unknown function. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: focal adhesion; cytoskeleton; intracellular membrane-bound organelle; plasma membrane; cell cortex. Molecular Function: actin binding. Biological Process: mitosis; cell division
size1 :
0.08 mL
price1 :
165 USD
size2 :
0.4 mL
price2 :
370
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
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