(Mouse) Tet1 Antibody (C-term) | MyBioSource MBS9206951 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

(Mouse) Tet1 Antibody (C-term)

catalog :

MBS9206951

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-Tet1 Antibody (C-term)at 1:2000 dilution + F9 whole cell lysates. Lysates/proteins at 20 ug per lane. Secondary. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 219 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

product information

catalog number :

MBS9206951

products type :

Antibody

products full name :

(Mouse) Tet1 Antibody (C-term)

products short name :

[Tet1]

other names :

[methylcytosine dioxygenase TET1 isoform 2; Methylcytosine dioxygenase TET1; methylcytosine dioxygenase TET1; tet methylcytosine dioxygenase 1; CXXC-type zinc finger protein 6; Ten-eleven translocation 1 gene protein homolog]

products gene name :

[Tet1]

other gene names :

[Tet1; Tet1; LCX; Cxxc6; AA517754; BB001228; mKIAA1676; D10Ertd17e; 2510010B09Rik; Cxxc6; Kiaa1676]

uniprot entry name :

TET1_MOUSE

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Mouse

sequence length :

2007

specificity :

This mouse Tet1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1845-1879 amino acids from the C-terminal region of mouse Tet1.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Store at 2-8°C for up to 2 weeks. For long term storage store at -20°C in smalls aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA)

app notes :

WB~~1:2000

image1 heading :

Western Blot (WB)

other info1 :

Calculated MW: 219261

other info2 :

Function: Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5- hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Also mediates subsequent conversion of 5hmC into 5- formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probablyconstitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, plays a more general role in chromatin regulation. Preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and Polycomb-repressed genes. Involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Also involved in transcription repression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells it plays a role in embryonic stem cells maintenance and inner cell mass cell specification. Plays an essential role in the tumorigenicity of glioblastoma cells. TET1-mediated production of 5hmC acts as a recruitment signal for the CHTOP-methylosome complex to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis (PubMed: 25284789 ). Cellular location: Nucleus. Tissue location: Present in embryonic stem cells (ES cells)

ncbi gi num :

241896976

ncbi acc num :

NP_081660.1

ncbi gb acc num :

NM_027384.1

uniprot acc num :

Q3URK3

ncbi pathways :

D-Glutamine And D-glutamate Metabolism Pathway (52463); D-Glutamine And D-glutamate Metabolism Pathway (52463); D-Glutamine And D-glutamate Metabolism Pathway (52463); D-Glutamine And D-glutamate Metabolism Pathway (52463)

uniprot summary :

TET1: Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5- hydroxymethylcytosine (5hmC). Might initiate a process leading to cytosine demethylation through deamination into 5- hydroxymethyluracil (5hmU) and subsequent replacement by unmethylated cytosine by the base excision repair system. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. Preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and polycomb-repressed genes. By controlling the levels of 5mC and 5hmC at gene promoters, it may regulate the gene expression silencing induced by cytosine methylation. May have a dual function by also repressing the expression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells it plays a role in embryonic stem cells maintenance and inner cell mass cell specification. A chromosomal aberration involving TET1 may be a cause of acute leukemias. Translocation t(10;11)(q22;q23) with MLL. This is a rare chromosomal translocation 5 MLL-TET1 3 . Belongs to the TET family. Protein type: EC 1.14.11.n2; DNA-binding; Oxidoreductase. Cellular Component: nucleus. Molecular Function: protein binding; structure-specific DNA binding; DNA binding; dioxygenase activity; zinc ion binding; iron ion binding; metal ion binding; oxidoreductase activity. Biological Process: protein amino acid O-linked glycosylation; inner cell mass cell differentiation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; stem cell maintenance; positive regulation of transcription from RNA polymerase II promoter; chromatin modification

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage