PCNA Antibody (C-term) | MyBioSource MBS9206515 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

PCNA Antibody (C-term)

catalog :

MBS9206515

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of PCNA Antibody (C-term) in Jurkat, Hela, 293 cell line lysates (35ug/lane). PCNA (arrow) was detected using the purified Pab.

image 2 :

Western blot analysis of PCNA (arrow) using rabbit polyclonal PCNA Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PCNA gene.

image 3 :

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PCNA antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

product information

catalog number :

MBS9206515

products type :

Antibody

products full name :

PCNA Antibody (C-term)

products short name :

[PCNA]

products name syn :

[Proliferating cell nuclear antigen; PCNA; Cyclin; PCNA]

other names :

[proliferating cell nuclear antigen; Proliferating cell nuclear antigen; proliferating cell nuclear antigen; proliferating cell nuclear antigen; Cyclin]

products gene name :

[PCNA]

other gene names :

[PCNA; PCNA; ATLD2; PCNA]

uniprot entry name :

PCNA_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human (Predicted Reactivity: Bovine, Hamster, Monkey, Rat)

sequence positions :

[231-261]

sequence length :

261

specificity :

This PCNA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-261 amino acids from the C-terminal region of human PCNA.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

concentration :

Vial Concentration: 2

storage stability :

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB)

app notes :

WB~~1:1000. IHC-P~~1:10~50. IF~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunofluorescence (IF)

image4 description :

Confocal immunofluorescent analysis of PCNA Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

image5 heading :

Immunofluorescence (IF)

image5 description :

Confocal immunofluorescent analysis of PCNA Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

other info1 :

Antigen Type: Synthetic Peptide. Precautions: PCNA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

other info2 :

Antigen Source: HUMAN. Function: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. Cellular Location: Nucleus. Note=Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902). Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.

products categories :

Cancer; Cell Biology

products description :

PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.

products references :

Wang,Y., J. Cell. Biochem. 106 (3), 409-413 (2009) Maga,G., Proc. Natl. Acad. Sci. U.S.A. 105 (52), 20689-20694 (2008) Acharya,N., Proc. Natl. Acad. Sci. U.S.A. 105 (46), 17724-17729 (2008)

ncbi gi num :

4505641

ncbi acc num :

NP_002583.1

ncbi gb acc num :

NM_002592.2

uniprot acc num :

P12004

ncbi mol weight :

28769

ncbi summary :

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

uniprot summary :

PCNA: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase s processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3 - 5 exonuclease and 3 -phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Homotrimer. Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with p21Cip1/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Interacts with DDX11. Interacts with EGFR; positively regulates PCNA. Interacts with C12orf48/PARI. Interacts with SMARCAD1. Belongs to the PCNA family. Protein type: Cell cycle regulation; DNA replication. Chromosomal Location of Human Ortholog: 20pter-p12. Cellular Component: nucleoplasm; centrosome; PCNA complex; cytoplasm; nuclear replication fork; DNA replication factor C complex; nucleus. Molecular Function: identical protein binding; protein binding; DNA polymerase processivity factor activity; MutLalpha complex binding; purine-specific mismatch base pair DNA N-glycosylase activity; dinucleotide insertion or deletion binding; receptor tyrosine kinase binding. Biological Process: mismatch repair; heart development; positive regulation of deoxyribonuclease activity; DNA strand elongation during DNA replication; response to lipid; leading strand elongation; DNA repair; telomere maintenance via semi-conservative replication; G1/S-specific transcription in mitotic cell cycle; cell proliferation; bypass DNA synthesis; response to cadmium ion; epithelial cell differentiation; base-excision repair; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; telomere maintenance; regulation of DNA replication; G1/S transition of mitotic cell cycle. Disease: Ataxia-telangiectasia-like Disorder 2

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage