CLNS1A Antibody (C-term); Methylosome subunit pICln; Chloride channel; nucleotide sensitive 1A; Chloride conductance regulatory protein ICln; I(Cln); Chloride ion current inducer protein; ClCI; Reticulocyte pICln; CLNS1A; CLCI; ICLN | MyBioSource MBS9206277 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CLNS1A Antibody (C-term); Methylosome subunit pICln; Chloride channel; nucleotide sensitive 1A; Chloride conductance regulatory protein ICln; I(Cln); Chloride ion current inducer protein; ClCI; Reticulocyte pICln; CLNS1A; CLCI; ICLN

catalog :

MBS9206277

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, flow cytometry, FACS, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

All lanes : Anti-CLNS1A Antibody (C-term) at 1:8000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

image 2 :

Western blot analysis of CLNS1A antibody (C-term) in HL60 cell line lysates (35ug/lane). CLNS1A (arrow) was detected using the purified Pab.

image 3 :

Formalin-fixed and paraffin-embedded human colon carcinoma with CLNS1A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

product information

catalog number :

MBS9206277

products type :

Antibody

products full name :

products short name :

[CLNS1A]

products name syn :

[CLCI, ICLN]

other names :

[methylosome subunit pICln; Methylosome subunit pICln; methylosome subunit pICln; chloride channel, nucleotide-sensitive, 1A; Chloride channel, nucleotide sensitive 1A; Chloride conductance regulatory protein ICln; I(Cln); Chloride ion current inducer protein; ClCI; Reticulocyte pICln]

products gene name :

[CLNS1A]

other gene names :

[CLNS1A; CLNS1A; CLCI; ICln; CLNS1B; CLCI; ICLN; I(Cln); ClCI]

uniprot entry name :

ICLN_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human (Predicted Reactivity: Rabbit)

sequence positions :

[189-216]

sequence length :

237

specificity :

This CLNS1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-216 amino acids from the C-terminal region of human CLNS1A.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles

tested application :

Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin(IHC-P), Flow Cytometry (FC/FACS)

app notes :

WB~~1:1000. FC~~1:10~50. IHC-P~~1:50~100

image1 heading :

Test Data

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Flow Cytometry (FC/FACS)

image4 description :

Flow cytometric analysis of hela cells using CLNS1A Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

other info1 :

Function: Chaperone that regulates the assembly of spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. May also indirectly participate in cellular volume control by activation of a swelling-induced chloride conductance pathway. Cellular Location: Cytoplasm, cytosol. Nucleus. Cytoplasm, cytoskeleton. Note=A small fraction is also associated with the cytoskeleton

products categories :

Cardiovascular

products description :

CLNS1A is a protein that functions in multiple regulatory pathways. The protein complexes with numerous cytosolic proteins and performs diverse functions including regulation of small nuclear ribonucleoprotein biosynthesis, platelet activation and cytoskeletal organization. The protein is also found associated with the plasma membrane where it functions as a chloride current regulator.

products references :

Yeung,C.H., Biol. Reprod. 73 (5), 1057-1063 (2005)

ncbi gi num :

4502891

ncbi acc num :

NP_001284.1

ncbi gb acc num :

NM_001293.2

uniprot acc num :

P54105

ncbi mol weight :

26215

ncbi pathways :

Ribosome Pathway (1207); Ribosome Pathway (1207); Ribosome Pathway (1207); Ribosome Pathway (1207); Ribosome Pathway (1207)

ncbi summary :

This gene encodes a protein that functions in multiple regulatory pathways. The encoded protein complexes with numerous cytosolic proteins and performs diverse functions including regulation of small nuclear ribonucleoprotein biosynthesis, platelet activation and cytoskeletal organization. The protein is also found associated with the plasma membrane where it functions as a chloride current regulator. Pseudogenes of this gene are found on chromosomes 1, 4 and 6. [provided by RefSeq, Feb 2009]

uniprot summary :

ICLN: The interaction with Sm proteins inhibits their assembly on U RNA and interferes with snRNP biogenesis. Inhibits the binding of survival motor neuron protein (SMN) to Sm proteins. May participate in cellular volume control by activation of a swelling-induced chloride conductance pathway. Belongs to the pICln (TC 1.A.47) family. Protein type: Channel, chloride. Chromosomal Location of Human Ortholog: 11q13.5-q14. Cellular Component: nucleoplasm; cytoskeleton; cytoplasm; plasma membrane; cytosol; nucleus. Molecular Function: protein binding. Biological Process: spliceosomal snRNP biogenesis; gene expression; chloride transport; cell volume homeostasis

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage