antibody

Cleaved LC3B Antibody

MBS9205943

0.08 mL

155 USD

polyclonal

rabbit

nonconjugated

human, mouse

ELISA, immunocytochemistry, enzyme immunoassay

Antibody

Cleaved LC3B Antibody

Cleaved LC3B

Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta; MAP1LC3B; MAP1ALC3

microtubule-associated proteins 1A/1B light chain 3B; Microtubule-associated proteins 1A/1B light chain 3B; microtubule-associated proteins 1A/1B light chain 3B; microtubule-associated protein 1 light chain 3 beta; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta

MAP1LC3B

MAP1LC3B; MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3; MAP1ALC3; MAP1A/MAP1B LC3 B

MLP3B_HUMAN

Polyclonal

Rabbit Ig

Rabbit

Human, mouse (Predicted Reactivity: Bovine)

89-122

125

This Cleaved LC3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-122 amino acids from human Cleaved LC3B.

Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.25

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunofluorescence (IF), ELISA (EIA), Immunocytochemistry (ICC)

IF~~1:10~50. ICC~~1:10~50

Antigen Type: Synthetic Peptide. Other Accession: O41515

Antigen Source: HUMAN. Function : Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. Cellular Location: Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes Localizes also to discrete punctae along the ciliary axoneme (By similarity). Tissue Location: Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver

Cancer; Cardiovascular; Metabolism; Neuroscience; Signal Transduction; Autophagy

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

References for protein:. 1.Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005). 2.Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005). 3.Greenberg JT. Dev Cell. 8(6):799-801. (2005). 4. Levine B. Cell. 120(2):159-62. (2005). 5.Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004). 6.Tanida I., et al. Int. J. Biochem. Cell Biol. 36:2503-2518(2004). 7.He H., et al. J. Biol. Chem. 278:29278-29287(2003). 8.Tanida I., et al. J. Biol. Chem. 279:36268-36276(2004). References for U251 cell line:. 1. Westermark B.; Pontén J.; Hugosson R. (1973)." Determinants for the establishment of permanent tissue culture lines from human gliomas". Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449]. 2. Pontén, J.,Westermark B. (1978)." Properties of Human Malignant Glioma Cells in Vitro". Medical Biology 56: 184-193.[PMID: 359950]. 3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). "Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent". Neuro Oncol. 12(5): 473-481.[ PMID: 20406898].

12383056

NP_073729.1

NM_022818.4

Q9GZQ8

14688

Nitrotoluene Degradation Pathway (81631)

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]

LC3B: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Interacts at microtubules with CABP1 (via EF-hands 1 and 2) but not with calmodulin. Recruits proteins such as SQSTM1 and NBR1 to the autophagosome. Protein type: Vesicle; Ubiquitin-like modifier; Autophagy; Microtubule-binding. Chromosomal Location of Human Ortholog: 16q24.2. Cellular Component: microtubule; extrinsic to membrane; mitochondrion; endomembrane system; autophagic vacuole; intracellular; cytoplasmic vesicle; axoneme; cytosol. Molecular Function: protein binding; microtubule binding; GABA receptor binding. Biological Process: mitochondrion degradation; autophagic vacuole formation; cellular response to nitrogen starvation

0.08 mL

155 USD

0.4 mL

340