Human 3-oxo-5-alpha-steroid 4-dehydrogenase 2, SRD5A2 ELISA Kit | MyBioSource MBS920584 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human 3-oxo-5-alpha-steroid 4-dehydrogenase 2, SRD5A2 ELISA Kit

catalog :

MBS920584

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS920584

products type :

ELISA Kit

products full name :

Human 3-oxo-5-alpha-steroid 4-dehydrogenase 2, SRD5A2 ELISA Kit

products short name :

steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2)

products name syn :

Human 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5A2) ELISA kit; MGC138457; 3-oxo-5 alpha-steroid 4-dehydrogenase 2; steroid 5-alpha-reductase 2; steroid-5-alpha-reductase; alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2)

other names :

3-oxo-5-alpha-steroid 4-dehydrogenase 2; 3-oxo-5-alpha-steroid 4-dehydrogenase 2; 3-oxo-5-alpha-steroid 4-dehydrogenase 2; S5AR 2; SR type 2; 5 alpha-SR2; type II 5-alpha reductase; steroid 5-alpha-reductase 2; steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2); 5 alpha-SR2; SR type 2; Steroid 5-alpha-reductase 2; S5AR 2; Type II 5-alpha reductase

products gene name :

SRD5A2

other gene names :

SRD5A2; SRD5A2; S5AR 2

uniprot entry name :

S5A2_HUMAN

reactivity :

Human

sequence length :

254

specificity :

This assay has high sensitivity and excellent specificity for detection of human SRD5A2. No significant cross-reactivity or interference between human SRD5A2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 12 pg/ml -2400 pg/ml. Sensitivity: 6 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for SRD5A2 and Horseradish Peroxidase (HRP) conjugated SRD5A2. The competitive inhibition reaction is launched between with HRP labeled SRD5A2 and unlabeled SRD5A2 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of SRD5A2 in the sample. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

39812447

ncbi acc num :

NP_000339.2

ncbi gb acc num :

NM_000348.3

uniprot acc num :

P31213

ncbi mol weight :

28,393 Da

ncbi pathways :

Androgen Biosynthesis Pathway (106154); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Metabolism Of Steroid Hormones And Vitamin D Pathway (106150); Prostate Cancer Pathway (83111); Prostate Cancer Pathway (523); Steroid Hormone Biosynthesis Pathway (82940); Steroid Hormone Biosynthesis Pathway (301); Androgen Biosynthesis Pathway (545302); Androgen Biosynthesis Pathway (545459)

ncbi summary :

This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH). [provided by RefSeq, Jul 2008]

uniprot summary :

SRD5A2: Converts testosterone (T) into 5-alpha- dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. Defects in SRD5A2 are the cause of pseudovaginal perineoscrotal hypospadias (PPSH). A form of male pseudohermaphroditism in which 46,XY males show ambiguous genitalia at birth, including perineal hypospadias and a blind perineal pouch, and develop masculinization at puberty. The name of the disorder stems from the finding of a blind-ending perineal opening resembling a vagina and a severely hypospadiac penis with the urethra opening onto the perineum. Belongs to the steroid 5-alpha reductase family. Protein type: Oxidoreductase; Membrane protein, multi-pass; EC 1.3.1.22; Lipid Metabolism - androgen and estrogen; Membrane protein, integral. Chromosomal Location of Human Ortholog: 2p23. Cellular Component: endoplasmic reticulum membrane; cell soma; integral to membrane. Molecular Function: amide binding; cholestenone 5-alpha-reductase activity; sterol 5-alpha reductase activity; 3-oxo-5-alpha-steroid 4-dehydrogenase activity. Biological Process: response to nutrient levels; steroid metabolic process; response to drug; response to peptide hormone stimulus; hypothalamus development; dibenzo-p-dioxin metabolic process; hippocampus development; androgen metabolic process; male gonad development; phthalate metabolic process; response to testosterone stimulus; androgen biosynthetic process; female genitalia development; biphenyl metabolic process; cell-cell signaling; male genitalia development; cell differentiation; response to follicle-stimulating hormone stimulus; steroid catabolic process. Disease: Pseudovaginal Perineoscrotal Hypospadias

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage