Human Corticotropin-releasing factor-binding protein, CRHBP ELISA Kit | MyBioSource MBS920581 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Corticotropin-releasing factor-binding protein, CRHBP ELISA Kit

catalog :

MBS920581

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS920581

products type :

ELISA Kit

products full name :

Human Corticotropin-releasing factor-binding protein, CRHBP ELISA Kit

products short name :

corticotropin releasing hormone binding protein

products name syn :

Human Corticotropin-releasing factor-binding protein (CRHBP) ELISA kit; CRF-BP; CRFBP; corticotropin releasing hormone-binding protein; corticotropin releasing hormone binding protein

other names :

corticotropin-releasing factor-binding protein; Corticotropin-releasing factor-binding protein; corticotropin-releasing factor-binding protein; CRH-BP; CRF-binding protein; corticotropin releasing hormone-binding protein; corticotropin-releasing hormone-binding protein; corticotropin releasing hormone binding protein; Corticotropin-releasing hormone-binding protein; CRH-BP

products gene name :

CRHBP

other gene names :

CRHBP; CRHBP; CRFBP; CRF-BP; CRFBP; CRF-BP; CRF-binding protein; CRH-BP

uniprot entry name :

CRHBP_HUMAN

reactivity :

Human

sequence length :

322

specificity :

This assay has high sensitivity and excellent specificity for detection of human CRHBP. No significant cross-reactivity or interference between human CRHBP and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of human CRHBP is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CRHBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CRHBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CRHBP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CRHBP bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

47080099

ncbi acc num :

NP_001873.2

ncbi gb acc num :

NM_001882.3

uniprot acc num :

P24387

ncbi mol weight :

36,144 Da

ncbi pathways :

Corticotropin-releasing Hormone Pathway (920957)

ncbi summary :

Corticotropin-releasing hormone is a potent stimulator of synthesis and secretion of preopiomelanocortin-derived peptides. Although CRH concentrations in the human peripheral circulation are normally low, they increase throughout pregnancy and fall rapidly after parturition. Maternal plasma CRH probably originates from the placenta. Human plasma contains a CRH-binding protein which inactivates CRH and which may prevent inappropriate pituitary-adrenal stimulation in pregnancy. [provided by RefSeq, Jul 2008]

uniprot summary :

CRHBP: Binds CRF and inactivates it. May prevent inappropriate pituitary-adrenal stimulation in pregnancy. Belongs to the CRF-binding protein family. Protein type: Secreted, signal peptide; Secreted; Inhibitor. Chromosomal Location of Human Ortholog: 5q13.3. Cellular Component: multivesicular body; Golgi apparatus; extracellular space; microtubule; endoplasmic reticulum; dendrite; perikaryon; intracellular; nerve terminal; nucleus; secretory granule. Molecular Function: protein binding; corticotropin-releasing hormone binding; peptide binding. Biological Process: negative regulation of adrenocorticotropic hormone secretion; synaptic transmission, dopaminergic; regulated secretory pathway; learning and/or memory; hormone-mediated signaling; cellular response to stress; regulation of adrenocorticotropin hormone secretion; hormone metabolic process; female pregnancy; behavioral response to ethanol; signal transduction; inflammatory response

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage