PLA2G7 Antibody (Center) | MyBioSource MBS9205733 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

PLA2G7 Antibody (Center)

catalog :

MBS9205733

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, flow cytometry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Staining PLA2G7 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

image 2 :

staining PLA2G7 in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

image 3 :

Overlay histogram showing HL-60 cells stained with MBS9205733 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MBS9205733, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1?g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

product information

catalog number :

MBS9205733

products type :

Antibody

products full name :

PLA2G7 Antibody (Center)

products short name :

[PLA2G7]

products name syn :

[Platelet-activating factor acetylhydrolase; PAF acetylhydrolase; 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; Group-VIIA phospholipase A2; gVIIA-PLA2; LDL-associated phospholipase A2; LDL-PLA(2); PAF 2-acylhydrolase; PLA2G7; PAFAH]

other names :

[platelet-activating factor acetylhydrolase; Platelet-activating factor acetylhydrolase; platelet-activating factor acetylhydrolase; phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma); 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; Group-VIIA phospholipase A2; gVIIA-PLA2; LDL-associated phospholipase A2; LDL-PLA(2); PAF 2-acylhydrolase]

products gene name :

[PLA2G7]

other gene names :

[PLA2G7; PLA2G7; PAFAD; PAFAH; LP-PLA2; LDL-PLA2; PAFAH; PAF acetylhydrolase; gVIIA-PLA2; LDL-PLA(2)]

uniprot entry name :

PAFA_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human

sequence positions :

[200-228]

sequence length :

441

specificity :

This PLA2G7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-228 amino acids from the Central region of human PLA2G7.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

IHC-P, FC, WB, ELISA

app notes :

WB~~1:1000. IHC-P~~1:10~50. FC~~1:25

image1 heading :

IHC-P

image2 heading :

IHC-P

image3 heading :

Testing Data

image4 heading :

Western Blot

image4 description :

All lanes : Anti-PLA2G7 Antibody (Center) at 1:2000 dilution Lane 1: human liver lysate Lane 2: human lung lysate Lane 3: human spleen lysate Lane 4: human plasma lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

image5 heading :

Western Blot

image5 description :

Western blot analysis of PLA2G7 Antibody (Center) (Cat. #MBS9205733) in HL-60 cell line lysates (35ug/lane). PLA2G7 (arrow) was detected using the purified Pab.

other info1 :

Function: Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biolocially inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.

other info2 :

Cellular Location: Secreted, extracellular space. Tissue Location: Plasma.

products categories :

Cardiovascular; Immunology

products description :

products references :

Fan, P., et al. Hum. Reprod. 25(5):1288-1294(2010). Paik, J.K., et al. Clin. Chim. Acta 411 (7-8), 486-493 (2010). Meng, X., et al. Psychiatry Res 175 (1-2), 186-187 (2010). McGeachie, M., et al. Circulation 120(24):2448-2454(2009). Cojocaru, I.M., et al. Rom J Intern Med 47(1):61-65(2009)

ncbi gi num :

270133071

ncbi acc num :

NP_001161829.1

ncbi gb acc num :

NM_001168357.1

uniprot acc num :

Q13093

ncbi summary :

The protein encoded by this gene is a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. Defects in this gene are a cause of platelet-activating factor acetylhydrolase deficiency. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Dec 2009]

uniprot summary :

PLA2G7: Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids. Defects in PLA2G7 are the cause of platelet-activating factor acetylhydrolase deficiency (PAFAD). An enzymatic deficiency that results in exacerbated bodily response to inflammatory agents. Asthmatic individuals affected by this condition may manifest severe respiratory symptoms. Defects in PLA2G7 are a cause of susceptibility to asthma (ASTHMA). The most common chronic disease affecting children and young adults. It is a complex genetic disorder with a heterogeneous phenotype, largely attributed to the interactions among many genes and between these genes and the environment. It is characterized by recurrent attacks of paroxysmal dyspnea, with weezing due to spasmodic contraction of the bronchi. PLA2G7 variants can be a risk factor for the development of asthma and PLA2G7 may act as a modifier gene that modulates the severity of this disease. Defects in PLA2G7 are a cause of susceptibility to atopic hypersensitivity (ATOPY). A condition characterized by predisposition to develop hypersensitivity reactions. Atopic individuals can develop eczema, allergic rhinitis and allergic asthma. Belongs to the AB hydrolase superfamily. Lipase family. Protein type: Secreted, signal peptide; Hydrolase; EC 3.1.1.47; Secreted; Lipid Metabolism - ether lipid. Chromosomal Location of Human Ortholog: 6p21.2-p12. Cellular Component: cytoplasm; extracellular region. Molecular Function: calcium-independent phospholipase A2 activity; phospholipid binding; 1-alkyl-2-acetylglycerophosphocholine esterase activity. Biological Process: cellular protein metabolic process; lipid catabolic process; positive regulation of inflammatory response. Disease: Asthma, Susceptibility To; Ige Responsiveness, Atopic; Platelet-activating Factor Acetylhydrolase Deficiency

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage