LIF Antibody (Center) | MyBioSource MBS9205709 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

LIF Antibody (Center)

catalog :

MBS9205709

quantity :

0.08 mL

price :

150 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-LIF Antibody (Center) at 1:1000 dilution + Rat spleen lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa . Blocking/Dilution buffer: 5% NFDM/TBST.

image 2 :

All lanes : Anti- LIF Antibod y (Center) at 1:1000 dilution . Lane 1: KG-1 whole cell lysate . Lane 2: Human spleen whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa . Blocking/Dilution buffer: 5% NFDM/TBST.

product information

catalog number :

MBS9205709

products type :

Antibody

products full name :

LIF Antibody (Center)

products short name :

[LIF]

products name syn :

[Leukemia inhibitory factor; LIF; Differentiation-stimulating factor; D factor; Melanoma-derived LPL inhibitor; MLPLI; Emfilermin; LIF; HILDA]

other names :

[leukemia inhibitory factor isoform 2; Leukemia inhibitory factor; leukemia inhibitory factor; leukemia inhibitory factor; Differentiation-stimulating factor; D factor; Melanoma-derived LPL inhibitor; MLPLI; INN: Emfilermin]

products gene name :

[LIF]

other gene names :

[LIF; LIF; CDF; DIA; HILDA; MLPLI; HILDA; LIF; D factor; MLPLI]

uniprot entry name :

LIF_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human

sequence positions :

[72-101]

sequence length :

specificity :

This LIF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-101 amino acids from the Central region of human LIF.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

concentration :

Vial Concentration: 0.5

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

app notes :

WB~~1:1000

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot

other info1 :

Antigen Type: Synthetic Peptide

other info2 :

Antigen Source: HUMAN

products categories :

Cancer; Cell Biology; Immunology; Neuroscience; Signal Transduction

products description :

LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.

products references :

Novotny,Z., et.al., Folia Biol. (Praha) 55 (3), 92-97 (2009)

ncbi gi num :

380418324

ncbi acc num :

NP_001244064.1

ncbi gb acc num :

NM_001257135.1

uniprot acc num :

P15018

ncbi mol weight :

22008

ncbi summary :

The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]

uniprot summary :

LIF: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. Belongs to the LIF/OSM family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 22q12.2. Cellular Component: extracellular space; cytoplasm. Molecular Function: growth factor activity; leukemia inhibitory factor receptor binding; cytokine activity; receptor binding. Biological Process: transcription from RNA polymerase II promoter; leukemia inhibitory factor signaling pathway; multicellular organismal development; negative regulation of hormone secretion; stem cell maintenance; tyrosine phosphorylation of Stat3 protein; decidualization; positive regulation of peptidyl-serine phosphorylation; positive regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of MAPKKK cascade; negative regulation of meiosis; positive regulation of cell proliferation; neuron development; immune response; positive regulation of transcription from RNA polymerase II promoter; blood vessel remodeling; positive regulation of macrophage differentiation; muscle morphogenesis; alveolus development; embryo implantation; positive regulation of peptidyl-serine phosphorylation of STAT protein; positive regulation of astrocyte differentiation

size1 :

0.08 mL

price1 :

150 USD

more info or order :

MyBioSource product webpage