Mouse Fgfr4 Antibody (Center) | MyBioSource MBS9205497 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Fgfr4 Antibody (Center)

catalog :

MBS9205497

quantity :

0.08 mL

price :

150 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

mouse, rat

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

All lanes : Anti-Fgfr4 Antibody (Center) at 1:2000 dilution. Lane 1: mouse skeletal muscle lysates. Lane 2: mouse spleen lysates. Lane 3: rat lung lysates. Lane 4: mouse lung lysates. Lysates/proteins at 20 ug per lane. Secondary. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 89 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

product information

catalog number :

MBS9205497

products type :

Antibody

products full name :

Mouse Fgfr4 Antibody (Center)

products short name :

[Fgfr4]

products name syn :

[Fibroblast growth factor receptor 4; FGFR-4; Protein-tyrosine kinase receptor MPK-11; CD334; Fgfr4; Fgfr-4; Mpk-11]

other names :

[fibroblast growth factor receptor 4; Fibroblast growth factor receptor 4; fibroblast growth factor receptor 4; fibroblast growth factor receptor 4; Protein-tyrosine kinase receptor MPK-11; CD_antigen: CD334]

products gene name :

[Fgfr4]

other gene names :

[Fgfr4; Fgfr4; Fgfr-4; Fgfr-4; Mpk-11; FGFR-4]

uniprot entry name :

FGFR4_MOUSE

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Mouse, rat

sequence length :

799

specificity :

This Mouse Fgfr4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 448-482 amino acids from the Central region of Mouse Fgfr4.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

concentration :

Vial Concentration: 0.5

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA)

app notes :

WB~~1:2000

image1 heading :

Western Blot (WB)

other info1 :

Crown Antibody: Yes. Clone Names: RB50681

other info2 :

Antigen Source: HUMAN. Cellular Location: Cell membrane; Single-pass type I membrane protein Endosome. Endoplasmic reticulum Note=Internalized from the cell membrane to recycling endosomes, and from there back to the cell membrane. Tissue Location: Isoform 1 and isoform 2 are expressed in lung and proliferating myoblasts and myotubes of primary myogenic cells (at protein level). Isoform 1 and isoform 2 are expressed in liver, muscle, spleen, heart, lung, kidney and in primary myogenic cells.

products categories :

Signal Transduction; Neuroscience; Immunology; Cell Biology; Cancer; Crown Antibodies; Amyotrophic Lateral Sclerosis (ALS)

products description :

Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Plays a role in postnatal lung development. May be involved in the development of skeletal muscle cell lineages.

products references :

Stark K.L.,et al.Development 113:641-651(1991). Kwiatkowski B.A.,et al.J. Cell. Physiol. 215:803-817(2008). Carninci P.,et al.Science 309:1559-1563(2005). Gilardi-Hebenstreit P.,et al.Oncogene 7:2499-2506(1992). Weinstein M.,et al.Development 125:3615-3623(1998).

ncbi gi num :

112293260

ncbi acc num :

NP_032037.2

ncbi gb acc num :

NM_008011.2

uniprot acc num :

Q03142

ncbi mol weight :

88661

ncbi pathways :

, Pathway (14186); , Pathway (14186); , Pathway (14186); , Pathway (14186); , Pathway (14186); , Pathway (14186); , Pathway (14186); , Pathway (14186); , Pathway (14186); , Pathway (14186)

uniprot summary :

FGFR4: a tyrosine-kinase receptor that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of the MAP kinase and AKT signaling pathways. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling. Two isoforms of the human protein are formed by alternative splicing. This description may include information from UniProtKB. Protein type: Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); Kinase, protein; EC 2.7.10.1; TK group; FGFR family. Cellular Component: nucleoplasm; Golgi apparatus; transport vesicle; membrane; endoplasmic reticulum; integral to plasma membrane; cytoplasm; integral to membrane; plasma membrane; intercellular junction; endosome. Molecular Function: transferase activity; heparin binding; protein binding; fibroblast growth factor binding; fibroblast growth factor receptor activity; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding; protein kinase activity. Biological Process: cell migration; fibroblast growth factor receptor signaling pathway; regulation of lipid metabolic process; peptidyl-tyrosine phosphorylation; positive regulation of proteolysis; protein amino acid autophosphorylation; positive regulation of metalloenzyme activity; glucose homeostasis; protein amino acid phosphorylation; induction of an organ; positive regulation of cell proliferation; phosphate ion homeostasis; phosphorylation; lung development

size1 :

0.08 mL

price1 :

150 USD

more info or order :

MyBioSource product webpage