Phospho-PI3KC3 (S164) Antibody | MyBioSource MBS9205320 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Phospho-PI3KC3 (S164) Antibody

catalog :

MBS9205320

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

ELISA, enzyme immunoassay, dot blot

more info or order :

MyBioSource product webpage

image

image 1 :

Dot blot analysis of Phospho-PI3KC3-S164 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

product information

catalog number :

MBS9205320

products type :

Antibody

products full name :

Phospho-PI3KC3 (S164) Antibody

products short name :

[Phospho-PI3KC3 (S164)]

products name syn :

[Phosphatidylinositol 3-kinase catalytic subunit type 3; PI3-kinase type 3; PI3K type 3; PtdIns-3-kinase type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide-3-kinase class 3; hVps34; PIK3C3; VPS34]

other names :

[phosphatidylinositol 3-kinase catalytic subunit type 3 isoform 1; Phosphatidylinositol 3-kinase catalytic subunit type 3; phosphatidylinositol 3-kinase catalytic subunit type 3; phosphatidylinositol 3-kinase, catalytic subunit type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide-3-kinase class 3; hVps34]

products gene name :

[PIK3C3]

other gene names :

[PIK3C3; PIK3C3; VPS34; Vps34; hVps34; VPS34; PI3-kinase type 3; PI3K type 3; PtdIns-3-kinase type 3]

uniprot entry name :

PK3C3_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human (Predicted Reactivity: Mouse, Pig, Rat)

sequence length :

887

specificity :

This PI3KC3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S164 of human PI3KC3.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Dot Blot (DB), ELISA (EIA)

app notes :

DB~~1:500

image1 heading :

Dot Blot (DB)

products categories :

Immunology; Metabolism; Signal Transduction; Phospho Antibodies

products description :

PI3KC3 is a catalytic subunit of the PI3K complex involved in the transport of lysosomal enzyme precursors to lysosomes. This enzyme acts catalytically to convert 1-phosphatidyl-1D-myo-inositol to 1-phosphatidyl-1D-myo-inositol 3-phosphate. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.

products references :

Vergne, I., et al., J. Exp. Med. 198(4):653-659 (2003). Volinia, S., et al., EMBO J. 14(14):3339-3348 (1995).

ncbi gi num :

34761064

ncbi acc num :

NP_002638.2

ncbi gb acc num :

NM_002647.3

uniprot acc num :

Q8NEB9

ncbi mol weight :

101549

ncbi pathways :

Taurine And Hypotaurine Metabolism Pathway (5289); Taurine And Hypotaurine Metabolism Pathway (5289); Taurine And Hypotaurine Metabolism Pathway (5289); Taurine And Hypotaurine Metabolism Pathway (5289); Taurine And Hypotaurine Metabolism Pathway (5289); Taurine And Hypotaurine Metabolism Pathway (5289); Taurine And Hypotaurine Metabolism Pathway (5289); Taurine And Hypotaurine Metabolism Pathway (5289); Taurine And Hypotaurine Metabolism Pathway (5289); Taurine And Hypotaurine Metabolism Pathway (5289)

uniprot summary :

Function: Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed: 20643123 , PubMed: 20208530 ). Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes (By similarity). Cellular Location: Midbody. Late endosome. Cytoplasmic vesicle, autophagosome. Note=As component of the PI3K complex I localized to pre-autophagosome structures. As component of the PI3K complex II localized predominantly to endosomes (Probable). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity).{ECO:0000250 UniProtKB:Q6PF93,ECO:0000305 PubMed:14617358}. Tissue Location: Ubiquitously expressed, with a highest expression in skeletal muscle.

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage