product summary
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company name :
MyBioSource
product type :
antibody
product name :
NRK Antibody (Center)
catalog :
MBS9204967
quantity :
0.08 mL
price :
165 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunocytochemistry, enzyme immunoassay
more info or order :
image
image 1 :
NRK Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NRK antibody detected the NRK protein (arrow).
image 2 :
Fluorescent confocal image of Hela cell stained with NRK Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NRK primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NRK immunoreactivity is localized to Cytoplasm and Nucleus significantly.
product information
catalog number :
MBS9204967
products type :
Antibody
products full name :
NRK Antibody (Center)
products short name :
[NRK]
products name syn :
[Nik-related protein kinase; NRK]
other names :
[nik-related protein kinase; Nik-related protein kinase; nik-related protein kinase; Nik related kinase]
products gene name :
[NRK]
other gene names :
[NRK; NRK; NESK]
uniprot entry name :
NRK_HUMAN
clonality :
Polyclonal
isotype :
Rabbit Ig
host :
Rabbit
reactivity :
Human
sequence positions :
[721-750]
sequence length :
188
specificity :
This NRK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 721-750 amino acids from the Central region of human NRK.
purity :
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
form :
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
concentration :
Vial Concentration: 0.5
storage stability :
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
tested application :
Western Blot (WB),Immunofluorescence (IF), ELISA (EIA)
app notes :
WB~~1:1000. IF~~1:10~50
image1 heading :
Western Blot (WB)
image2 heading :
Immunofluorescence (IF)
other info1 :
Antigen Type: Synthetic Peptide
other info2 :
Antigen Source: HUMAN. Function: May phosphorylate cofilin-1 and induce actin polymerization through this process, during the late stages of embryogenesis. Involved in the TNF-alpha-induced signaling pathway (By similarity).
products categories :
Signal Transduction
products description :
The mouse ortholog of this gene encodes a protein kinase required for JNK activation. The encoded protein may be involved in the induction of actin polymerization in late embryogenesis.
products references :
Ross, M.T., et al. Nature 434(7031):325-337(2005). Nakano, K., et al. Exp. Cell Res. 287(2):219-227(2003)
ncbi gi num :
118600963
ncbi acc num :
NP_940867.2
ncbi gb acc num :
NM_198465.3
uniprot acc num :
Q7Z2Y5
ncbi mol weight :
178479
ncbi pathways :
, Pathway (203447)
ncbi summary :
The mouse ortholog of this gene encodes a protein kinase required for JNK activation. The encoded protein may be involved in the induction of actin polymerization in late embryogenesis.[provided by RefSeq, Jun 2010]
uniprot summary :
NRK: May phosphorylate cofilin-1 and induce actin polymerization through this process, during the late stages of embryogenesis. Involved in the TNF-alpha-induced signaling pathway. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; EC 2.7.11.1; Kinase, protein; STE group; STE20 family; MSN subfamily. Chromosomal Location of Human Ortholog: Xq22.3. Cellular Component: cytoplasm. Molecular Function: ATP binding; receptor signaling protein serine/threonine kinase activity. Biological Process: negative regulation of cell proliferation; parturition; protein amino acid phosphorylation; activation of JNKK activity
size1 :
0.08 mL
price1 :
165 USD
size2 :
0.4 mL
price2 :
370
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
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