Leptin Receptor (LEPR) Antibody (N-term) | MyBioSource MBS9204850 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Leptin Receptor (LEPR) Antibody (N-term)

catalog :

MBS9204850

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, flow cytometry, FACS, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Leptin Receptor (LEPR) Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the LEPR antibody detected the LEPR protein (arrow).

image 2 :

Leptin Receptor (LEPR) Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

image 3 :

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

product information

catalog number :

MBS9204850

products type :

Antibody

products full name :

Leptin Receptor (LEPR) Antibody (N-term)

products short name :

[Leptin Receptor (LEPR)]

products name syn :

[Leptin receptor; LEP-R; HuB219; OB receptor; OB-R; CD295; LEPR; DB; OBR]

other names :

[leptin receptor isoform 3; Leptin receptor; leptin receptor; leptin receptor; HuB219; OB receptor; OB-R; CD_antigen: CD295]

products gene name :

[LEPR]

other gene names :

[LEPR; LEPR; OBR; OB-R; CD295; LEP-R; LEPRD; DB; OBR; LEP-R; OB-R]

uniprot entry name :

LEPR_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human

sequence positions :

[23-52]

sequence length :

839

specificity :

This Leptin Receptor (LEPR) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-52 amino acids from the N-terminal region of human Leptin Receptor (LEPR).

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry-Paraffin (IHC-P)

app notes :

WB~~1:1000. FC~~1:10~50. IHC-P~~1:50~100

image1 heading :

Western Blot (WB)

image2 heading :

Flow Cytometry (FC/FACS)

image3 heading :

Immunohistochemistry (IHC)

other info1 :

Calculated MW: 132494

other info2 :

Function: Receptor for hormone LEP/leptin (Probable) (PubMed:22405007). On ligand binding, mediates LEP central and peripheral effects through the activation of different signaling pathways such as JAK2/STAT3 and MAPK cascade/FOS. In the hypothalamus, LEP acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones (By similarity) (PubMed:9537324). In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic and affects innate and adaptive immunity (PubMed:25060689, PubMed:12504075, PubMed:8805376). Control of energy homeostasis and melanocortin production (stimulation of POMC and full repression of AgRP transcription) is mediated by STAT3 signaling, whereas distinct signals regulate NPY and the control of fertility, growth and glucose homeostasis. Involved in the regulation of counter- regulatory response to hypoglycemia by inhibiting neurons of the parabrachial nucleus. Has a specific effect on T lymphocyte responses, differentially regulating the proliferation of naive and memory T -ells. Leptin increases Th1 and suppresses Th2 cytokine production (By similarity). Cellular location: Cell membrane; Single-pass type I membrane protein. Basolateral cell membrane. Tissue Location: Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary Low level in lung and kidney. Isoform B is highly expressed in hypothalamus, but also in skeletal muscle. Detected in fundic and antral epithelial cells of the gastric mucosa (PubMed:19159218) Isoform B and isoform A are expressed by NK cells (at protein level) (PubMed:12504075).

products categories :

Cancer; Cardiovascular; Metabolism; Neuroscience; Signal Transduction

products description :

Leptin, an adipocyte-specific hormone, regulates adipose-tissue mass through hypothalamic effects on satiety and energy expenditure by acting through the leptin receptor (LEPR). LEPR is a single-transmembrane-domain receptor of the cytokine receptor family that is identical to the mouse diabetes (db) gene product. During weight loss, leptin levels decrease, whereas soluble LEPR levels and the receptor bound fraction of leptin increases. The presence of LEPR in the absorptive cells of the small intestine suggests that leptin may have a physiological role in the regulation of nutrient absorption.

products references :

Schroth, M., et al., J. Clin. Endocrinol. Metab. 88(11):5497-5501 (2003). Couturier, C., et al., J. Biol. Chem. 278(29):26604-26611 (2003). Gavrila, A., et al., J. Clin. Endocrinol. Metab. 88(6):2838-2843 (2003). Yannakoulia, M., et al., J. Clin. Endocrinol. Metab. 88(4):1730-1736 (2003). Kado, N., et al., Hum. Reprod. 18(4):715-720 (2003).

ncbi gi num :

51093379

ncbi acc num :

NP_001003679.1

ncbi gb acc num :

NM_001003679.3

uniprot acc num :

P48357

ncbi pathways :

, Pathway (3953); , Pathway (3953); , Pathway (3953); , Pathway (3953); , Pathway (3953); , Pathway (3953); , Pathway (3953); , Pathway (3953); , Pathway (3953); , Pathway (3953)

ncbi summary :

The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010]

uniprot summary :

LEPR: the receptor for leptin (obesity factor). A single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Five alternatively spliced transcript variants have been reported. Protein type: Membrane protein, integral; Receptor, cytokine. Chromosomal Location of Human Ortholog: 1p31. Cellular Component: plasma membrane; extracellular region; integral to membrane; receptor complex. Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; identical protein binding; protein binding; transmembrane receptor activity. Biological Process: cholesterol metabolic process; cell surface receptor linked signal transduction; cytokine and chemokine mediated signaling pathway; multicellular organismal development; negative regulation of gluconeogenesis; negative regulation of hydrolase activity; energy reserve metabolic process. Disease: Leptin Receptor Deficiency

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage