ELISA/assay

Dog Pyruvate kinase isozymes R/L, PKLR ELISA Kit

MBS920482

48-Strip-Wells

565 USD

ELISA Kit

Dog Pyruvate kinase isozymes R/L, PKLR ELISA Kit

pyruvate kinase, liver and RBC

Dog Pyruvate kinase isozymes R/L (PKLR) ELISA kit; PK1; PKL; PKR; PKRL; RPK; R-type/L-type pyruvate kinase; pyruvate kinase 1; pyruvate kinase isozyme R/L; pyruvate kinase type L; pyruvate kinase; liver and blood cell; red cell/liver pyruvate kinase; pyruvate kinase; liver and RBC

pyruvate kinase PKLR isoform 2; Pyruvate kinase PKLR; pyruvate kinase PKLR; Pyruvate kinase isozyme R; Pyruvate kinase isozymes R/L; pyruvate kinase isozymes L/R; Pyruvate kinase isozymes L/R

PKLR

PKLR; PKLR

KPYR_CANFA

Dog

543

This assay has high sensitivity and excellent specificity for detection of dog PKLR. No significant cross-reactivity or interference between dog PKLR and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 7.81 mU/ml-500 mU/ml. Sensitivity: The minimum detectable dose of dog PKLR is typically less than 1.95 mU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PKLR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PKLR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PKLR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PKLR bound in the initial step. The color development is stopped and the intensity of the color is measured.

365192553

NP_001242947.1

NM_001256018.1

Q29536

62,048 Da

Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway (617528); Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway (468242); Biosynthesis Of Amino Acids Pathway (790335); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (815245); Carbon Metabolism Pathway (817567); ChREBP Activates Metabolic Gene Expression Pathway (1017371); Developmental Biology Pathway (1016805); Disease Pathway (1016552); Glucose Metabolism Pathway (1016659)

Function: Plays a key role in glycolysis . Catalytic activity: ATP + pyruvate = ADP + phosphoenolpyruvate. Cofactor: Magnesium . Enzyme regulation: Allosterically activated by fructose 1,6-bisphosphate

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150