FGFR1 Antibody (Center) | MyBioSource MBS9204812 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

FGFR1 Antibody (Center)

catalog :

MBS9204812

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

[RB50848]

reactivity :

human, mouse, rat

application :

western blot, ELISA, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Flow cytometric analysis of SH-SY5Y cells using FGFR1 Antibody (Center)(green) compared to an isotype control of rabbit IgG(blue). MBS9204812 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

image 2 :

Western blot analysis of lysates from Hela, HepG2, MCF-7, SH-SY5Y, mouse NIH/3T3 cell line (from left to right), using FGFR1 Antibody (Center). MBS9204812 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

product information

catalog number :

MBS9204812

products type :

Antibody

products full name :

FGFR1 Antibody (Center)

products short name :

[FGFR1]

products name syn :

[Fibroblast growth factor receptor 1; FGFR-1; Basic fibroblast growth factor receptor 1; BFGFR; bFGF-R-1; Fms-like tyrosine kinase 2; FLT-2; N-sam; Proto-oncogene c-Fgr; CD331; FGFR1; BFGFR; CEK; FGFBR; FLG; FLT2; HBGFR]

other names :

[fibroblast growth factor receptor 1 isoform 10; Fibroblast growth factor receptor 1; fibroblast growth factor receptor 1; fibroblast growth factor receptor 1; Basic fibroblast growth factor receptor 1; BFGFR; bFGF-R-1; Fms-like tyrosine kinase 2; FLT-2; N-sam; Proto-oncogene c-Fgr; CD_antigen: CD331]

products gene name :

[FGFR1]

other gene names :

[FGFR1; FGFR1; CEK; FLG; HH2; OGD; FLT2; KAL2; BFGFR; CD331; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; HRTFDS; bFGF-R-1; BFGFR; CEK; FGFBR; FLG; FLT2; HBGFR; FGFR-1; BFGFR; bFGF-R-1; FLT-2]

uniprot entry name :

FGFR1_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

clone :

[RB50848]

host :

Rabbit

reactivity :

Human, mouse (Predicted Reactivity: Chicken, Rat)

sequence length :

853

specificity :

This FGFR1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 291-324 amino acids from the Central region of human FGFR1.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)

app notes :

FC~~1:25. WB~~1:1000

image1 heading :

Flow Cytometry (FC/FACS)

image2 heading :

Western Blot (WB)

other info2 :

Cellular Location : Cell membrane; Single-pass type I membrane protein. Nucleus. Cytoplasm, cytosol. Cytoplasmic vesicle Note=After ligand binding, both receptor and ligand are rapidly internalized. Can translocate to the nucleus after internalization, or by translocation from the endoplasmic reticulum or Golgi apparatus to the cytosol, and from there to the nucleus. Tissue Location: Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells.

products categories :

Crown Antibodies; Cancer; Cell Biology

products description :

Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation.

products references :

Itoh N.,et al.Biochem. Biophys. Res. Commun. 169:680-685(1990). Dionne C.A.,et al.EMBO J. 9:2685-2692(1990). Johnson D.E.,et al.Mol. Cell. Biol. 10:4728-4736(1990). Isacchi A.,et al.Nucleic Acids Res. 18:1906-1906(1990). Wennstroem S.,et al.Growth Factors 4:197-208(1991).

ncbi gi num :

291327489

ncbi acc num :

NP_001167534.1

ncbi gb acc num :

NM_001174063.1

uniprot acc num :

P11362

ncbi mol weight :

91868

ncbi pathways :

D-Glutamine And D-glutamate Metabolism Pathway (2260); D-Glutamine And D-glutamate Metabolism Pathway (2260); D-Glutamine And D-glutamate Metabolism Pathway (2260); D-Glutamine And D-glutamate Metabolism Pathway (2260); D-Glutamine And D-glutamate Metabolism Pathway (2260); D-Glutamine And D-glutamate Metabolism Pathway (2260); D-Glutamine And D-glutamate Metabolism Pathway (2260); D-Glutamine And D-glutamate Metabolism Pathway (2260); D-Glutamine And D-glutamate Metabolism Pathway (2260); D-Glutamine And D-glutamate Metabolism Pathway (2260)

ncbi summary :

The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations in this gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, and autosomal dominant Kallmann syndrome 2. Chromosomal aberrations involving this gene are associated with stem cell myeloproliferative disorder and stem cell leukemia lymphoma syndrome. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]

uniprot summary :

FGFR1: a receptor tyrosine kinase of the highly-conserved fibroblast growth factor receptor (FGFR). Binds both acidic and basic fibroblast growth factors and is involved in limb induction. Point mutations cause Pfeffer syndrome (finger and toe malformations and other skeletal errors) and dominant Kallmann syndrome 2. Stem cell leukemia lymphoma syndrome (SCLL) may be caused by a t(8;13)(p12;q12) translocation that fuses a zinc finger gene, ZNF198, to FGFR1. Various myeloproliferative disorders have been linked to translocations that fuse FGFR1 to FOP, FIM, CEP1 or the atypical kinase, Bcr. Inhibitor: SU5402. 20 isoforms of the human protein produced by alternative splicing have been described. Protein type: Kinase, protein; Membrane protein, integral; Protein kinase, tyrosine (receptor); Protein kinase, TK; Oncoprotein; EC 2.7.10.1; TK group; FGFR family. Chromosomal Location of Human Ortholog: 8p11.23-p11.22. Cellular Component: integral to plasma membrane; cytoplasmic membrane-bound vesicle; plasma membrane; integral to membrane; extracellular region; cytosol; nucleus; receptor complex. Molecular Function: heparin binding; identical protein binding; protein binding; protein homodimerization activity; fibroblast growth factor binding; fibroblast growth factor receptor activity; protein-tyrosine kinase activity; ATP binding. Biological Process: paraxial mesoderm development; axon guidance; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; cell maturation; neuron migration; negative regulation of transcription from RNA polymerase II promoter; middle ear morphogenesis; protein amino acid phosphorylation; positive regulation of MAP kinase activity; sensory perception of sound; positive regulation of MAPKKK cascade; ureteric bud development; induction of an organ; regulation of cell differentiation; midbrain development; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; chondrocyte differentiation; angiogenesis; skeletal development; embryonic limb morphogenesis; positive regulation of cardiac muscle cell proliferation; epidermal growth factor receptor signaling pathway; inner ear morphogenesis; cell migration; chordate embryonic development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; transcription, DNA-dependent; in utero embryonic development; outer ear morphogenesis; MAPKKK cascade; positive regulation of cell cycle; positive regulation of phosphoinositide 3-kinase cascade; neuroblast division in the ventricular zone; mesenchymal cell differentiation; skeletal morphogenesis; insulin receptor signaling pathway; innate immune response; auditory receptor cell development; positive regulation of neuron differentiation; regulation of lateral mesodermal cell fate specification. Disease: Pfeiffer Syndrome; Hypogonadotropic Hypogonadism 2 With Or Without Anosmia; Jackson-weiss Syndrome; Trigonocephaly 1; Osteoglophonic Dysplasia

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage