GARP Antibody (Center) | MyBioSource MBS9204616 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

GARP Antibody (Center)

catalog :

MBS9204616

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of GARP antibody (Center) in CEM cell line lysates (35ug/lane). GARP (arrow) was detected using the purified Pab.

image 2 :

Western blot analysis of GARP antibody (Center) in mouse cerebellum tissue lysates (35ug/lane). GARP (arrow) was detected using the purified Pab.

image 3 :

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with GARP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

product information

catalog number :

MBS9204616

products type :

Antibody

products full name :

GARP Antibody (Center)

products short name :

[GARP]

products name syn :

[Leucine-rich repeat-containing protein 32; Garpin; Glycoprotein A repetitions predominant; GARP; LRRC32; D11S833E; GARP]

other names :

[leucine-rich repeat-containing protein 32; Leucine-rich repeat-containing protein 32; leucine-rich repeat-containing protein 32; leucine rich repeat containing 32; Garpin; Glycoprotein A repetitions predominant; GARP]

products gene name :

[LRRC32]

other gene names :

[LRRC32; LRRC32; GARP; D11S833E; D11S833E; GARP; GARP]

uniprot entry name :

LRC32_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human, mouse

sequence positions :

[234-260]

sequence length :

662

specificity :

This GARP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 234-260 amino acids from the Central region of human GARP.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)

app notes :

WB~~1:1000. IHC-P~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with GARP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

image5 heading :

Immunohistochemistry (IHC)

image5 description :

Formalin-fixed and paraffin-embedded human cerebellum reacted with GARP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

other info1 :

Cellular Location: Cell membrane; Single-pass type I membrane protein. Cell surface. Tissue Location: Preferentially expressed in regulatory T-cells (T(regs)).

products description :

Background: GARP is a type I membrane protein which contains 20 leucine-rich repeats. Function: Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space. Associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta. Able to outcompete LTBP1 for binding to LAP regulatory chain of TGF-beta. Controls activation of TGF-beta-1 (TGFB1) on the surface of activated regulatory T-cells (Tregs). Required for epithelial fusion during palate development by regulating activation of TGF-beta-3 (TGFB3) (By similarity).

products references :

Wang,R., PLoS ONE 3 (7), E2705 (2008). Maire,G., Genes Chromosomes Cancer 37 (4), 389-395 (2003)

ncbi gi num :

193083139

ncbi acc num :

NP_001122394.1

ncbi gb acc num :

NM_001128922.1

uniprot acc num :

Q14392

ncbi mol weight :

71979

ncbi summary :

This gene encodes a type I membrane protein which contains 20 leucine-rich repeats. Alterations in the chromosomal region 11q13-11q14 are involved in several pathologies. [provided by RefSeq, Jul 2008]

uniprot summary :

LRRC32: . Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 11q13.5-q14. Cellular Component: integral to plasma membrane. Biological Process: negative regulation of activated T cell proliferation; negative regulation of cytokine secretion

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage