CD19 Antibody (N-term) | MyBioSource MBS9204578 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CD19 Antibody (N-term)

catalog :

MBS9204578

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, flow cytometry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of lysate from Ramos cell line, using CD19Antibody (N-term). MBS9204578 was diluted at 1:500. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

image 2 :

CD19 Antibody (N-term) western blot analysis in Raji cell line lysates (35ug/lane).This demonstrates the CD19 antibody detected the CD19 protein (arrow).

image 3 :

Western blot analysis of CD19 (arrow) using rabbit polyclonal CD19 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD19 gene (Lane 2) (Origene Technologies).

product information

catalog number :

MBS9204578

products type :

Antibody

products full name :

CD19 Antibody (N-term)

products short name :

[CD19]

products name syn :

[B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD19; CD19]

other names :

[B-lymphocyte antigen CD19 isoform 1; B-lymphocyte antigen CD19; B-lymphocyte antigen CD19; CD19 molecule; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD_antigen: CD19]

products gene name :

[CD19]

other gene names :

[CD19; CD19; B4; CVID3]

uniprot entry name :

CD19_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human

sequence positions :

[143-172]

sequence length :

557

specificity :

This CD19 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 143-172 amino acids from the N-terminal region of human CD19.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC), Immunohistochemistry-Paraffin (IHC-P), Elisa (EIA)

app notes :

WB~~1:1000. IF~~1:10~50. FC~~1:10~50. IHC-P~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Western Blot (WB)

image4 heading :

Immunofluorescence (IF)

image4 description :

Immunofluorescence analysis of CD19 Antibody (N-term) with paraffin-embedded human lymph tissue. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.

image5 heading :

Flow Cytometry (FC/FACS)

image5 description :

Flow cytometric analysis of CEM cells using CD19 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

other info2 :

Function: Assembles with the antigen receptor ofB-lymphocytes in order to decrease thethreshold for antigen receptor-dependentstimulation. Cellular Location: Membrane; Single-pass type I membrane protein

products categories :

Crown Antibodies; Cancer; Immunology; Signal Transduction

products description :

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

products references :

Deaglio,S., Blood 109 (12), 5390-5398 (2007). Bradbury,L.E., J. Immunol. 149 (9), 2841-2850 (1992). Kozmik,Z., Mol. Cell. Biol. 12 (6), 2662-2672 (1992)

ncbi gi num :

296010921

ncbi acc num :

NP_001171569.1

ncbi gb acc num :

NM_001178098.1

uniprot acc num :

P15391

ncbi summary :

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008]

uniprot summary :

CD19: a cell surface molecule which assembles with the antigen receptor of B lymphocytes. A critical signal transduction molecule that regulates B lymphocyte development, activation, and differentiation. Ligation increases intracellular calcium levels and decreases the threshold for antigen receptor signaling. Germinal center formation is significantly reduced in CD19-deficient mice. Protein type: Membrane protein, integral; Cell surface. Chromosomal Location of Human Ortholog: 16p11.2. Cellular Component: protein complex; integral to plasma membrane; plasma membrane; external side of plasma membrane. Molecular Function: receptor signaling protein activity. Biological Process: epidermal growth factor receptor signaling pathway; regulation of immune response; B cell receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; cell surface receptor linked signal transduction; nerve growth factor receptor signaling pathway; cellular defense response; innate immune response; positive regulation of release of sequestered calcium ion into cytosol. Disease: Immunodeficiency, Common Variable, 2; Immunodeficiency, Common Variable, 3

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage