FAT10 Antibody (C-term) | MyBioSource MBS9204261 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

FAT10 Antibody (C-term)

catalog :

MBS9204261

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

FAT10 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the hFAT10 antibody detected the hFAT10 protein (arrow).

image 2 :

FAT10 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FAT10 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

image 3 :

Confocal immunofluorescent analysis of FAT10 Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

product information

catalog number :

MBS9204261

products type :

Antibody

products full name :

FAT10 Antibody (C-term)

products short name :

[FAT10]

products name syn :

[Ubiquitin D; Diubiquitin; Ubiquitin-like protein FAT10; UBD; FAT10]

other names :

[ubiquitin D; Ubiquitin D; ubiquitin D; ubiquitin D; Diubiquitin; Ubiquitin-like protein FAT10]

products gene name :

[UBD]

other gene names :

[UBD; UBD; FAT10; UBD-3; GABBR1; FAT10]

uniprot entry name :

UBD_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Mouse

sequence positions :

[120-153]

sequence length :

165

specificity :

This FAT10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120-153 amino acids from the C-terminal region of human FAT10.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

app notes :

WB~~1:1000. IHC-P~~1:50~100. IF~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Calculated MW: 18473

other info2 :

Cellular Location: Nucleus. Cytoplasm Note=Accumulates in aggresomes under proteasome inhibition conditions. Tissue Location: Constitutively expressed in mature dendritic cells and B-cells. Mostly expressed in the reticuloendothelial system (e.g. thymus, spleen), the gastrointestinal system, kidney, lung and prostate gland.

products categories :

Cell Biology; Immunology; Microbiology; Signal Transduction

products references :

Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010). Frank, B., et al. Int. J. Cancer (2010) In press :. Gong, P., et al. J. Am. Soc. Nephrol. 21(2):316-326(2010). Castellanos-Rubio, A., et al. Hum. Immunol. 71(1):96-99(2010). Barcellos, L.F., et al. PLoS Genet. 5 (10), E1000696 (2009) :

ncbi gi num :

222352096

ncbi acc num :

NP_006389.2

ncbi gb acc num :

NM_006398.3

uniprot acc num :

O15205

uniprot summary :

FAT10: Ubiquitin-like protein modifier which can be covalently attached to target protein and subsequently leads to their degradation by the 26S proteasome, in a NUB1L-dependent manner. Probably functions as a survival factor. Conjugation ability activated by UBA6. Promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2). Regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha. Required for TNF-alpha-induced p65 nuclear translocation in renal tubular epithelial cells (RTECs). May be involved in dendritic cell (DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T-cell responses. Mediates mitotic non-disjunction and chromosome instability, in long-term in vitro culture and cancers, by abbreviating mitotic phase and impairing the kinetochore localization of MAD2L1 during the prometaphase stage of the cell cycle. May be involved in the formation of aggresomes when proteasome is saturated or impaired. Mediates apoptosis in a caspase-dependent manner, especially in renal epithelium and tubular cells during renal diseases such as polycystic kidney disease and Human immunodeficiency virus (HIV)- associated nephropathy (HIVAN). Protein type: Ubiquitin-like modifier. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: cytoplasm; nucleus. Molecular Function: protein binding. Biological Process: ubiquitin-dependent protein catabolic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein modification by small protein conjugation; positive regulation of apoptosis; protein ubiquitination; myeloid dendritic cell differentiation; response to organic nitrogen; proteolysis

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage