ELP2 Antibody (C-term) | MyBioSource MBS9204251 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ELP2 Antibody (C-term)

catalog :

MBS9204251

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Fluorescent confocal image of SY5Y cells stained with ELP2 (C-term) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9204251 ELP2 (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the ELP2 immunosignal mainly to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000134759).

image 2 :

Western blot analysis of ELP2 antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). ELP2 (arrow) was detected using the purified Pab.

image 3 :

Flow cytometric analysis of NCI-H292 cells using ELP2 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

product information

catalog number :

MBS9204251

products type :

Antibody

products full name :

ELP2 Antibody (C-term)

products short name :

[ELP2]

products name syn :

[Elongator complex protein 2; ELP2; SHINC-2; STAT3-interacting protein 1; StIP1; ELP2; STATIP1]

other names :

[elongator complex protein 2 isoform 1; Elongator complex protein 2; elongator complex protein 2; elongator acetyltransferase complex subunit 2; SHINC-2; STAT3-interacting protein 1; StIP1]

products gene name :

[ELP2]

other gene names :

[ELP2; ELP2; StIP; SHINC-2; STATIP1; STATIP1; ELP2; StIP1]

uniprot entry name :

ELP2_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human

sequence positions :

[737-765]

sequence length :

756

specificity :

This ELP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 737-765 amino acids from the C-terminal region of human ELP2.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Immunofluorescence (IF), ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)

app notes :

IF~~1:100. WB~~1:1000. FC~~1:10~50

image1 heading :

Immunofluorescence (IF)

image2 heading :

Western Blot (WB)

image3 heading :

Flow Cytometry (FC/FACS)

other info1 :

Function: Regulates the ligand-dependent activation of STAT3. Cellular Location: Cytoplam. Nucleus.

products categories :

Signal Transduction

products description :

ELP2 regulates the ligand-dependent activation of STAT3. The protein acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4.

products references :

References for protein:. 1.Kim,J.H., Proc. Natl. Acad. Sci. U.S.A. 99 (3), 1241-1246 (2002). 2.Hawkes,N.A., J. Biol. Chem. 277 (4), 3047-3052 (2002). References for SY5Y (SH-SY5Y; ATCC#CRL-2266): 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704].

ncbi gi num :

338968885

ncbi acc num :

NP_001229804.1

ncbi gb acc num :

NM_001242875.1

uniprot acc num :

Q6IA86

ncbi mol weight :

92500

ncbi pathways :

Tyrosine Metabolism Pathway (55250); Tyrosine Metabolism Pathway (55250); Tyrosine Metabolism Pathway (55250)

uniprot summary :

ELP2: Regulates the ligand-dependent activation of STAT3. Belongs to the WD repeat ELP2 family. 5 isoforms of the human protein are produced by alternative splicing. Chromosomal Location of Human Ortholog: 18q12.2. Cellular Component: transcription elongation factor complex; Elongator holoenzyme complex; cytoplasm; histone acetyltransferase complex. Molecular Function: protein kinase binding. Biological Process: regulation of transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; regulation of JAK-STAT cascade; RNA elongation from RNA polymerase II promoter

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage