ATG7 Antibody (C-term) | MyBioSource MBS9204169 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ATG7 Antibody (C-term)

catalog :

MBS9204169

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

All lanes : Anti-ATG7 Antibody (C-term) at1:500 dilution Lane 1: Caki-1 whole cell lysateLane 2: Hela whole cell lysate Lane 3: HepG2whole cell lysate Lysates/proteins at 40 µg perlane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 78 kDa Blocking/Dilutionbuffer: 5% NFDM/TBST.

image 2 :

All lanes : Anti-ATG7 Antibody (C-term) at1:1000 dilution Lane 1: HepG2 whole cell lysateLane 2: NIH/3T3 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidaseconjugated at 1/10000 dilution. Predicted bandsize : 78 kDa Blocking/Dilution buffer: 5%

image 3 :

Immunofluorescent analysis of U251 cells, using ATG7 Antibody (C-term). U251 cells(right) were treated with Chloroquine (50 uM,16h). MBS9204169 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).

product information

catalog number :

MBS9204169

products type :

Antibody

products full name :

ATG7 Antibody (C-term)

products short name :

[ATG7]

products name syn :

[Ubiquitin-like modifier-activating enzyme ATG7; ATG12-activating enzyme E1 ATG7; Autophagy-related protein 7; APG7-like; hAGP7; Ubiquitin-activating enzyme E1-like protein; ATG7; APG7L]

other names :

[ubiquitin-like modifier-activating enzyme ATG7 isoform b; Ubiquitin-like modifier-activating enzyme ATG7; ubiquitin-like modifier-activating enzyme ATG7; autophagy related 7; ATG12-activating enzyme E1 ATG7; Autophagy-related protein 7; APG7-like; hAGP7; Ubiquitin-activating enzyme E1-like protein]

products gene name :

[ATG7]

other gene names :

[ATG7; ATG7; GSA7; APG7L; APG7-LIKE; APG7L; APG7-like; hAGP7]

uniprot entry name :

ATG7_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human, Mouse. Predicted reactivity: Chicken, Rat

sequence positions :

[540-569]

sequence length :

623

specificity :

This ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 540-569 amino acids from the C-terminal region of human ATG7.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

WB~~1:1000. IF~~1:25. IHC-P~~1:50~100

image1 heading :

Testing Data(TD)

image2 heading :

Testing Data (TD)

image3 heading :

Immunofluorescence (IF)

image4 heading :

Western Blot (WB)

image4 description :

Western blot analysis of APG7L Pab in 293 cell line lysate (35ug/lane). APG7L (arrow) was detected using the purified Pab.

image5 heading :

Immunohistochemistry (IHC)

image5 description :

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Autophagy APG7L antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

other info2 :

Function: E1-like activating enzyme involved in the 2ubiquitin- like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 s well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria o a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the aintenance of axonal homeostasis, the revention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. Cellular location: Cytoplasm. Preautophagosomal structure.Note=Localizes also to discrete punctaealong the ciliary axoneme and to the base ofthe ciliary axoneme. Tissue location: Widely expressed, especially in kidney, liver,lymph nodes and bone marrow.

products categories :

Signal Transduction; Metabolism; Developmental Biology; Cell Biology; Cardiovascular; Cancer; Phospho Antibodies

products description :

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.

products references :

References for protein:. 1.Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005). 2Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005). 3.Greenberg JT. Dev Cell. 8(6):799-801. (2005). 4.Levine B. Cell. 120(2):159-62. (2005). 5.Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004). 6.Tanida I., et al. Biochem. Biophys. Res. Commun. 292:256-262(2002). 7.Tanida I., et al. J. Biol. Chem. 277:13739-13744(2002). References for U251 cell line:. 1. Westermark B.; Pontén J.; Hugosson R. (1973)." Determinants for the establishment of permanent tissue culture lines from human gliomas". Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449]. 2. Pontén, J.,Westermark B. (1978)." Properties of Human Malignant Glioma Cells in Vitro". Medical Biology 56: 184-193.[PMID: 359950]. 3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). "Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent". Neuro Oncol. 12(5): 473-481.[ PMID: 20406898].

ncbi gi num :

222144227

ncbi acc num :

NP_001129503.2

ncbi gb acc num :

NM_001136031.2

uniprot acc num :

O95352

ncbi summary :

This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. [provided by RefSeq, Jan 2009]

uniprot summary :

ATG7: Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3. Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. Belongs to the ATG7 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Autophagy; Ubiquitin conjugating system. Chromosomal Location of Human Ortholog: 3p25.3. Cellular Component: cytoplasm; axoneme; cytosol. Molecular Function: protein binding; protein homodimerization activity; APG8 activating enzyme activity; APG12 activating enzyme activity; ubiquitin activating enzyme activity; transcription factor binding. Biological Process: regulation of protein ubiquitination; positive regulation of apoptosis; protein ubiquitination; pyramidal neuron development; adult walking behavior; post-embryonic development; protein transport; cardiac muscle cell development; protein amino acid lipidation; protein catabolic process; positive regulation of autophagy; amino acid metabolic process; central nervous system neuron axonogenesis; cerebellar Purkinje cell layer development; protein modification process; C-terminal protein lipidation; liver development; cellular response to starvation; cellular response to nitrogen starvation; neurological system process; protein modification by small protein conjugation; mitochondrion degradation; positive regulation of protein catabolic process; positive regulation of protein modification process; cerebral cortex development; positive regulation of macroautophagy; negative regulation of apoptosis

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage