FOXD1 Antibody (N-term) | MyBioSource MBS9204091 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

FOXD1 Antibody (N-term)

catalog :

MBS9204091

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

[RB34308]

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

All lanes : Anti-FOXD1 Antibody (N-term) at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: U-2OS whole cell lysate. Lane 3: U-87 MG whole cell lysate. Lane 4: Y79 whole cell lysate. Lane 5: Mouse kidney tissue lysate. Lane 6: Mouse eyeball tissue lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 46 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

image 2 :

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling FOXD1 with MBS9204091 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus staining on HepG2 cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red).

image 3 :

FOXD1 Antibody (N-term) (MBS9204091) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FOXD1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

product information

catalog number :

MBS9204091

products type :

Antibody

products full name :

FOXD1 Antibody (N-term)

products short name :

[FOXD1]

products name syn :

[Forkhead box protein D1; Forkhead-related protein FKHL8; Forkhead-related transcription factor 4; FREAC-4; FOXD1; FKHL8; FREAC4]

other names :

[forkhead box protein D1; Forkhead box protein D1; forkhead box protein D1; forkhead box D1; Forkhead-related protein FKHL8; Forkhead-related transcription factor 4; FREAC-4]

products gene name :

[FOXD1]

other gene names :

[FOXD1; FOXD1; FKHL8; FREAC4; FREAC-4; FKHL8; FREAC4; FREAC-4]

uniprot entry name :

FOXD1_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

clone :

[RB34308]

host :

Rabbit

reactivity :

Human, Mouse; Predicted: Mouse

sequence positions :

[1-30]

sequence length :

465

specificity :

This FOXD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human FOXD1.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

app notes :

IF~~1:25. WB~~1:2000. IHC-P~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

image3 heading :

Immunohistochemistry (IHC)

other info2 :

Function: Transcription factor required for formation of positional identity in the developing retina, regionalization of the optic chiasm and morphogenesis of the kidney. Can neuralize ectodermal cells directly (By similarity).Involved in transcriptional activation of PGF and C3 genes (PubMed: 27805902 ). Cellular location: Nucleus.

products description :

This intronless gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in tumor formation.

products references :

Berg, D.T., et al. J. Biol. Chem. 280(15):14943-14947(2005). Ernstsson, S., et al. J. Biol. Chem. 271(35):21094-21099(1996). Larsson, C., et al. Genomics 30(3):464-469(1995). Pierrou, S., et al. EMBO J. 13(20):5002-5012(1994)

ncbi gi num :

4758392

ncbi acc num :

NP_004463.1

ncbi gb acc num :

NM_004472.2

uniprot acc num :

Q16676

ncbi summary :

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. Studies of the orthologous mouse protein indicate that it functions in kidney development by promoting nephron progenitor differentiation, and it also functions in the development of the retina and optic chiasm. It may also regulate inflammatory reactions and prevent autoimmunity. [provided by RefSeq, Apr 2014]

uniprot summary :

FOXD1: Transcription factor required for formation of positional identity in the developing retina, regionalization of the optic chiasm and morphogenesis of the kidney. Can neuralize ectodermal cells directly. Protein type: DNA-binding; Motility/polarity/chemotaxis; Cell development/differentiation; Transcription factor. Chromosomal Location of Human Ortholog: 5q13.2. Cellular Component: nucleus. Molecular Function: DNA binding; sequence-specific DNA binding; transcription factor activity; DNA bending activity. Biological Process: axon guidance; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of BMP signaling pathway

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage