MFAP5 Antibody (C-term) | MyBioSource MBS9204084 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MFAP5 Antibody (C-term)

catalog :

MBS9204084

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, flow cytometry, FACS, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of MFAP5 antibody (C-term) in K562 cell line lysates (35ug/lane). MFAP5 (arrow) was detected using the purified Pab.

image 2 :

Formalin-fixed and paraffin-embedded human Skeletal muscle reacted with MFAP5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

image 3 :

MFAP5 Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

product information

catalog number :

MBS9204084

products type :

Antibody

products full name :

MFAP5 Antibody (C-term)

products short name :

[MFAP5]

products name syn :

[Microfibrillar-associated protein 5; MFAP-5; MP25; Microfibril-associated glycoprotein 2; MAGP-2; MFAP5; MAGP2]

other names :

[microfibrillar-associated protein 5 isoform a; Microfibrillar-associated protein 5; microfibrillar-associated protein 5; microfibrillar associated protein 5; MP25; Microfibril-associated glycoprotein 2; MAGP-2]

products gene name :

[MFAP5]

other gene names :

[MFAP5; MFAP5; AAT9; MP25; MAGP2; MAGP-2; MFAP-5; MAGP2; MFAP-5; MAGP-2]

uniprot entry name :

MFAP5_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human (Predicted Reactivity: Bovine, Mouse)

sequence positions :

[133-160]

specificity :

This MFAP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-160 amino acids from the C-terminal region of human MFAP5.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS)

app notes :

WB~~1:1000. IHC-P~~1:50~100. FC~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Flow Cytometry (FC/FACS)

image4 heading :

Western Blot (WB)

image4 description :

Western blot analysis of MFAP5 (arrow) using rabbit polyclonal MFAP5 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MFAP5 gene.

other info1 :

Function: May play a role in hematopoiesis. In the cardiovascular system, could regulate growth factors or participate in cell signaling in maintaining large vessel integrity (By similarity). Component of the elastin-associated microfibrils (PubMed: 8557636 ). Calculated MW: 19612

other info2 :

Cellular Location: Secreted, extracellular space, extracellular matrix

products categories :

Cancer; Signal Transduction

products description :

MFAP5 is a 25-kD microfibril-associated glycoprotein which is rich in serine and threonine residues. The protein lacks a hydrophobic carboxyl terminus and proline-, glutamine-, and tyrosine-rich regions, which are characteristics of a related 31-kDa microfibril-associated glycoprotein (MFAP2). The close similarity between these two proteins is confined to a central region of 60 aa where precise alignment of 7 cysteine residues occurs. The structural differences suggest that this protein has some functions that are distinct from those of MFAP2.

products references :

Albig,A.R., Becenti,D.J. Microvasc. Res. 76 (1), 7-14 (2008). Miyamoto,A., Lau,R. J. Biol. Chem. 281 (15), 10089-10097 (2006). Penner,A.S., Rock,M.J. J. Biol. Chem. 277 (38), 35044-35049 (2002). Hatzinikolas,G. J. Biol. Chem. 273 (45), 29309-29314 (1998)

ncbi gi num :

4505089

ncbi acc num :

NP_003471.1

ncbi gb acc num :

NM_003480.3

uniprot acc num :

Q13361

ncbi pathways :

, Pathway (8076); , Pathway (8076); , Pathway (8076); , Pathway (8076)

ncbi summary :

This gene encodes a 25-kD microfibril-associated glycoprotein which is a component of microfibrils of the extracellular matrix. The encoded protein promotes attachment of cells to microfibrils via alpha-V-beta-3 integrin. Deficiency of this gene in mice results in neutropenia. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]

uniprot summary :

MFAP5: Component of the elastin-associated microfibrils. Belongs to the MFAP family. Protein type: Secreted, signal peptide; Secreted; Extracellular matrix. Chromosomal Location of Human Ortholog: 12p13.1-p12.3. Cellular Component: microfibril; extracellular region. Molecular Function: extracellular matrix structural constituent. Biological Process: extracellular matrix organization and biogenesis; fibril organization and biogenesis. Disease: Aortic Aneurysm, Familial Thoracic 9

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage