product summary
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company name :
MyBioSource
product type :
antibody
product name :
MFAP5 Antibody (C-term)
catalog :
MBS9204084
quantity :
0.08 mL
price :
165 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, ELISA, flow cytometry, FACS, enzyme immunoassay, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
MyBioSource MBS9204084 image 1
Western blot analysis of MFAP5 antibody (C-term) in K562 cell line lysates (35ug/lane). MFAP5 (arrow) was detected using the purified Pab.
image 2 :
MyBioSource MBS9204084 image 2
Formalin-fixed and paraffin-embedded human Skeletal muscle reacted with MFAP5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
image 3 :
MyBioSource MBS9204084 image 3
MFAP5 Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
product information
catalog number :
MBS9204084
products type :
Antibody
products full name :
MFAP5 Antibody (C-term)
products short name :
[MFAP5]
products name syn :
[Microfibrillar-associated protein 5; MFAP-5; MP25; Microfibril-associated glycoprotein 2; MAGP-2; MFAP5; MAGP2]
other names :
[microfibrillar-associated protein 5 isoform a; Microfibrillar-associated protein 5; microfibrillar-associated protein 5; microfibrillar associated protein 5; MP25; Microfibril-associated glycoprotein 2; MAGP-2]
products gene name :
[MFAP5]
other gene names :
[MFAP5; MFAP5; AAT9; MP25; MAGP2; MAGP-2; MFAP-5; MAGP2; MFAP-5; MAGP-2]
uniprot entry name :
MFAP5_HUMAN
clonality :
Polyclonal
isotype :
Rabbit Ig
host :
Rabbit
reactivity :
Human (Predicted Reactivity: Bovine, Mouse)
sequence positions :
[133-160]
specificity :
This MFAP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-160 amino acids from the C-terminal region of human MFAP5.
purity :
Purified Rabbit Polyclonal Antibody (Pab)
form :
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
storage stability :
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
tested application :
Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS)
app notes :
WB~~1:1000. IHC-P~~1:50~100. FC~~1:10~50
image1 heading :
Western Blot (WB)
image2 heading :
Immunohistochemistry (IHC)
image3 heading :
Flow Cytometry (FC/FACS)
image4 heading :
Western Blot (WB)
image4 description :
Western blot analysis of MFAP5 (arrow) using rabbit polyclonal MFAP5 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MFAP5 gene.
other info1 :
Function: May play a role in hematopoiesis. In the cardiovascular system, could regulate growth factors or participate in cell signaling in maintaining large vessel integrity (By similarity). Component of the elastin-associated microfibrils (PubMed: 8557636 ). Calculated MW: 19612
other info2 :
Cellular Location: Secreted, extracellular space, extracellular matrix
products categories :
Cancer; Signal Transduction
products description :
MFAP5 is a 25-kD microfibril-associated glycoprotein which is rich in serine and threonine residues. The protein lacks a hydrophobic carboxyl terminus and proline-, glutamine-, and tyrosine-rich regions, which are characteristics of a related 31-kDa microfibril-associated glycoprotein (MFAP2). The close similarity between these two proteins is confined to a central region of 60 aa where precise alignment of 7 cysteine residues occurs. The structural differences suggest that this protein has some functions that are distinct from those of MFAP2.
products references :
Albig,A.R., Becenti,D.J. Microvasc. Res. 76 (1), 7-14 (2008). Miyamoto,A., Lau,R. J. Biol. Chem. 281 (15), 10089-10097 (2006). Penner,A.S., Rock,M.J. J. Biol. Chem. 277 (38), 35044-35049 (2002). Hatzinikolas,G. J. Biol. Chem. 273 (45), 29309-29314 (1998)
ncbi gi num :
4505089
ncbi acc num :
NP_003471.1
ncbi gb acc num :
NM_003480.3
uniprot acc num :
Q13361
ncbi pathways :
, Pathway (8076); , Pathway (8076); , Pathway (8076); , Pathway (8076)
ncbi summary :
This gene encodes a 25-kD microfibril-associated glycoprotein which is a component of microfibrils of the extracellular matrix. The encoded protein promotes attachment of cells to microfibrils via alpha-V-beta-3 integrin. Deficiency of this gene in mice results in neutropenia. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]
uniprot summary :
MFAP5: Component of the elastin-associated microfibrils. Belongs to the MFAP family. Protein type: Secreted, signal peptide; Secreted; Extracellular matrix. Chromosomal Location of Human Ortholog: 12p13.1-p12.3. Cellular Component: microfibril; extracellular region. Molecular Function: extracellular matrix structural constituent. Biological Process: extracellular matrix organization and biogenesis; fibril organization and biogenesis. Disease: Aortic Aneurysm, Familial Thoracic 9
size1 :
0.08 mL
price1 :
165 USD
size2 :
0.4 mL
price2 :
370
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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