catalog number :
MBS9204084
products full name :
MFAP5 Antibody (C-term)
products short name :
[MFAP5]
products name syn :
[Microfibrillar-associated protein 5; MFAP-5; MP25; Microfibril-associated glycoprotein 2; MAGP-2; MFAP5; MAGP2]
other names :
[microfibrillar-associated protein 5 isoform a; Microfibrillar-associated protein 5; microfibrillar-associated protein 5; microfibrillar associated protein 5; MP25; Microfibril-associated glycoprotein 2; MAGP-2]
products gene name :
[MFAP5]
other gene names :
[MFAP5; MFAP5; AAT9; MP25; MAGP2; MAGP-2; MFAP-5; MAGP2; MFAP-5; MAGP-2]
uniprot entry name :
MFAP5_HUMAN
reactivity :
Human (Predicted Reactivity: Bovine, Mouse)
sequence positions :
[133-160]
specificity :
This MFAP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-160 amino acids from the C-terminal region of human MFAP5.
purity :
Purified Rabbit Polyclonal Antibody (Pab)
form :
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
storage stability :
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
tested application :
Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS)
app notes :
WB~~1:1000. IHC-P~~1:50~100. FC~~1:10~50
image1 heading :
Western Blot (WB)
image2 heading :
Immunohistochemistry (IHC)
image3 heading :
Flow Cytometry (FC/FACS)
image4 heading :
Western Blot (WB)
image4 description :
Western blot analysis of MFAP5 (arrow) using rabbit polyclonal MFAP5 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MFAP5 gene.
other info1 :
Function: May play a role in hematopoiesis. In the cardiovascular system, could regulate growth factors or participate in cell signaling in maintaining large vessel integrity (By similarity). Component of the elastin-associated microfibrils (PubMed: 8557636 ). Calculated MW: 19612
other info2 :
Cellular Location: Secreted, extracellular space, extracellular matrix
products categories :
Cancer; Signal Transduction
products description :
MFAP5 is a 25-kD microfibril-associated glycoprotein which is rich in serine and threonine residues. The protein lacks a hydrophobic carboxyl terminus and proline-, glutamine-, and tyrosine-rich regions, which are characteristics of a related 31-kDa microfibril-associated glycoprotein (MFAP2). The close similarity between these two proteins is confined to a central region of 60 aa where precise alignment of 7 cysteine residues occurs. The structural differences suggest that this protein has some functions that are distinct from those of MFAP2.
products references :
Albig,A.R., Becenti,D.J. Microvasc. Res. 76 (1), 7-14 (2008). Miyamoto,A., Lau,R. J. Biol. Chem. 281 (15), 10089-10097 (2006). Penner,A.S., Rock,M.J. J. Biol. Chem. 277 (38), 35044-35049 (2002). Hatzinikolas,G. J. Biol. Chem. 273 (45), 29309-29314 (1998)
ncbi acc num :
NP_003471.1
ncbi gb acc num :
NM_003480.3
ncbi pathways :
, Pathway (8076); , Pathway (8076); , Pathway (8076); , Pathway (8076)
ncbi summary :
This gene encodes a 25-kD microfibril-associated glycoprotein which is a component of microfibrils of the extracellular matrix. The encoded protein promotes attachment of cells to microfibrils via alpha-V-beta-3 integrin. Deficiency of this gene in mice results in neutropenia. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]
uniprot summary :
MFAP5: Component of the elastin-associated microfibrils. Belongs to the MFAP family. Protein type: Secreted, signal peptide; Secreted; Extracellular matrix. Chromosomal Location of Human Ortholog: 12p13.1-p12.3. Cellular Component: microfibril; extracellular region. Molecular Function: extracellular matrix structural constituent. Biological Process: extracellular matrix organization and biogenesis; fibril organization and biogenesis. Disease: Aortic Aneurysm, Familial Thoracic 9