BACH2 Antibody (C-term) | MyBioSource MBS9203899 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

BACH2 Antibody (C-term)

catalog :

MBS9203899

quantity :

0.08 mL

price :

150 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, flow cytometry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Overlay histogram showing Ramos cells stained with MBS9203899 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MBS9203899, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

image 2 :

All lanes : Anti-BACH2 Antibody (C-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: Daudi whole cell lysate Lane 3: K562 whole cell lysate Lane 4: Ramos whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 93 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

image 3 :

BACH2 Antibody (C-term) (MBS9203899) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negativecontrol cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

product information

catalog number :

MBS9203899

products type :

Antibody

products full name :

BACH2 Antibody (C-term)

products short name :

[BACH2]

products name syn :

[Transcription regulator protein BACH2; BTB and CNC homolog 2; BACH2]

other names :

[transcription regulator protein BACH2; Transcription regulator protein BACH2; transcription regulator protein BACH2; BTB and CNC homology 1, basic leucine zipper transcription factor 2; BTB and CNC homolog 2]

products gene name :

[BACH2]

other gene names :

[BACH2; BACH2; BTBD25]

uniprot entry name :

BACH2_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human

specificity :

This BACH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 815-841 amino acids from the C-terminal region of human BACH2.

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Flow Cytometry (FC), Western Blot (WB), ELISA (EIA)

app notes :

FC~~1:10~50. WB~~1:2000

image1 heading :

Testing Data

image2 heading :

Testing Data

image3 heading :

Testing Data

other info1 :

Antigen Region: 815-841. Tissue Location: B-cell specific. Cellular Location: Cytoplasm. Nucleus; Note: Nucleocytoplasmic shuttling is controlled by phosphorylation. Function: Transcriptional regulator that acts as repressor or activator (By similarity). Binds to Maf recognition elements (MARE) (By similarity). Plays an important role in coordinating transcription activation and repression by MAFK (By similarity). Induces apoptosis in response to oxidative stress through repression of the antiapoptotic factor HMOX1. Positively regulates the nuclear import of actin (By similarity).

products categories :

Cell Biology; Immunology; Metabolism

products references :

Hinks, A., et al. Ann. Rheum. Dis. (2010) In press :. Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :. Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010). Pierce, B.L., et al. Hum. Hered. 69(3):193-201(2010). Barrett, J.C., et al. Nat. Genet. 41(6):703-707(2009)

ncbi gi num :

282847373

ncbi acc num :

NP_001164265.1

ncbi gb acc num :

NM_001170794.1

uniprot acc num :

Q9BYV9

uniprot summary :

BACH2: Transcriptional regulator that acts as repressor or activator. Binds to Maf recognition elements (MARE). Play important roles in coordinating transcription activation and repression by MAFK. Belongs to the bZIP family. CNC subfamily. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 6q15. Cellular Component: cytoplasm; nucleus. Biological Process: transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter

size1 :

0.08 mL

price1 :

150 USD

size2 :

0.4 mL

price2 :

340

more info or order :

MyBioSource product webpage