product summary
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company name :
MyBioSource
product type :
antibody
product name :
BACH2 Antibody (C-term)
catalog :
MBS9203899
quantity :
0.08 mL
price :
150 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, flow cytometry, enzyme immunoassay
more info or order :
image
image 1 :
MyBioSource MBS9203899 image 1
Overlay histogram showing Ramos cells stained with MBS9203899 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MBS9203899, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
image 2 :
MyBioSource MBS9203899 image 2
All lanes : Anti-BACH2 Antibody (C-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: Daudi whole cell lysate Lane 3: K562 whole cell lysate Lane 4: Ramos whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 93 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
image 3 :
MyBioSource MBS9203899 image 3
BACH2 Antibody (C-term) (MBS9203899) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negativecontrol cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
product information
catalog number :
MBS9203899
products type :
Antibody
products full name :
BACH2 Antibody (C-term)
products short name :
[BACH2]
products name syn :
[Transcription regulator protein BACH2; BTB and CNC homolog 2; BACH2]
other names :
[transcription regulator protein BACH2; Transcription regulator protein BACH2; transcription regulator protein BACH2; BTB and CNC homology 1, basic leucine zipper transcription factor 2; BTB and CNC homolog 2]
products gene name :
[BACH2]
other gene names :
[BACH2; BACH2; BTBD25]
uniprot entry name :
BACH2_HUMAN
clonality :
Polyclonal
isotype :
Rabbit Ig
host :
Rabbit
reactivity :
Human
specificity :
This BACH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 815-841 amino acids from the C-terminal region of human BACH2.
form :
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
storage stability :
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
tested application :
Flow Cytometry (FC), Western Blot (WB), ELISA (EIA)
app notes :
FC~~1:10~50. WB~~1:2000
image1 heading :
Testing Data
image2 heading :
Testing Data
image3 heading :
Testing Data
other info1 :
Antigen Region: 815-841. Tissue Location: B-cell specific. Cellular Location: Cytoplasm. Nucleus; Note: Nucleocytoplasmic shuttling is controlled by phosphorylation. Function: Transcriptional regulator that acts as repressor or activator (By similarity). Binds to Maf recognition elements (MARE) (By similarity). Plays an important role in coordinating transcription activation and repression by MAFK (By similarity). Induces apoptosis in response to oxidative stress through repression of the antiapoptotic factor HMOX1. Positively regulates the nuclear import of actin (By similarity).
products categories :
Cell Biology; Immunology; Metabolism
products references :
Hinks, A., et al. Ann. Rheum. Dis. (2010) In press :. Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :. Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010). Pierce, B.L., et al. Hum. Hered. 69(3):193-201(2010). Barrett, J.C., et al. Nat. Genet. 41(6):703-707(2009)
ncbi gi num :
282847373
ncbi acc num :
NP_001164265.1
ncbi gb acc num :
NM_001170794.1
uniprot acc num :
Q9BYV9
uniprot summary :
BACH2: Transcriptional regulator that acts as repressor or activator. Binds to Maf recognition elements (MARE). Play important roles in coordinating transcription activation and repression by MAFK. Belongs to the bZIP family. CNC subfamily. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 6q15. Cellular Component: cytoplasm; nucleus. Biological Process: transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter
size1 :
0.08 mL
price1 :
150 USD
size2 :
0.4 mL
price2 :
340
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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