ICOS Antibody (C-term) | MyBioSource MBS9203882 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ICOS Antibody (C-term)

catalog :

MBS9203882

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, flow cytometry, FACS, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Formalin-fixed and paraffin-embedded human lymph with ICOS Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

image 2 :

ICOS-S171 western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ICOS antibody detected the ICOS protein (arrow).

image 3 :

ICOS Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

product information

catalog number :

MBS9203882

products type :

Antibody

products full name :

ICOS Antibody (C-term)

products short name :

[ICOS]

products name syn :

[Inducible T-cell costimulator; Activation-inducible lymphocyte immunomediatory molecule; CD278; ICOS; AILIM]

other names :

[inducible T-cell costimulator; Inducible T-cell costimulator; inducible T-cell costimulator; inducible T-cell co-stimulator; Activation-inducible lymphocyte immunomediatory molecule; CD_antigen: CD278]

products gene name :

[ICOS]

other gene names :

[ICOS; ICOS; AILIM; CD278; CVID1; AILIM]

uniprot entry name :

ICOS_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human

sequence positions :

[156-185]

sequence length :

168

specificity :

This ICOS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-185 amino acids from the C-terminal region of human ICOS.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry-Paraffin (IHC-P)

app notes :

IHC-P~~1:50~100. WB~~1:1000. FC~~1:10~50

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

image3 heading :

Flow Cytometry (FC/FACS)

other info1 :

Function: Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up- regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up- regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre- activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes (By similarity).

other info2 :

Cellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Tissue Location: Activated T-cells. Highly expressed on tonsillar T-cells, which are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B- cell maturation. Expressed at lower levels in thymus, lung, lymph node and peripheral blood leukocytes. Expressed in the medulla of fetal and newborn thymus.

products categories :

Immunology

products description :

ICOS belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.

products references :

Okamoto, N., et al., Biochem. Biophys. Res. Commun. 310(3):691-702 (2003). Okamoto, T., et al., J. Rheumatol. 30(6):1157-1163 (2003). Riley, J.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(18):11790-11795 (2002). Haimila, K.E., et al., Immunogenetics 53(12):1028-1032 (2002). Witsch, E.J., et al., Eur. J. Immunol. 32(9):2680-2686 (2002).

ncbi gi num :

15029518

ncbi acc num :

NP_036224.1

ncbi gb acc num :

NM_012092.3

uniprot acc num :

Q9Y6W8

ncbi mol weight :

22625

ncbi pathways :

Valine, Leucine And Isoleucine Degradation Pathway (29851); Valine, Leucine And Isoleucine Degradation Pathway (29851); Valine, Leucine And Isoleucine Degradation Pathway (29851); Valine, Leucine And Isoleucine Degradation Pathway (29851); Valine, Leucine And Isoleucine Degradation Pathway (29851); Valine, Leucine And Isoleucine Degradation Pathway (29851); Valine, Leucine And Isoleucine Degradation Pathway (29851); Valine, Leucine And Isoleucine Degradation Pathway (29851); Valine, Leucine And Isoleucine Degradation Pathway (29851); Valine, Leucine And Isoleucine Degradation Pathway (29851)

ncbi summary :

The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation. [provided by RefSeq, Jul 2008]

uniprot summary :

ICOS: Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up- regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up- regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre- activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes. Defects in ICOS are the cause of immunodeficiency common variable type 1 (CVID1). CVID1 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be lo. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 2q33. Cellular Component: integral to plasma membrane; plasma membrane; extracellular region; external side of plasma membrane. Biological Process: cell-cell adhesion; T cell tolerance induction; T cell costimulation; immune response. Disease: Immunodeficiency, Common Variable, 2; Immunodeficiency, Common Variable, 1

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage