CLDN2 Antibody (C-term Y224) | MyBioSource MBS9203312 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CLDN2 Antibody (C-term Y224)

catalog :

MBS9203312

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, flow cytometry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-CLDN2 Anti-CLDN2 Antibody at 1:1000 dilution +human heart lysate Lysates/proteins at 20 µgper lane. Secondary Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated at 1/10000dilution. Predicted band size : 25 kDaBlocking/Dilution buffer: 5% NFDM/TBST.

image 2 :

Western blot analysis of CLDN2 (arrow) using rabbit polyclonal CLDN2 Antibody (Y224). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CLDN2 gene.

image 3 :

CLDN2 Antibody (C-term Y224) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CLDN2 Antibody (C-term Y224) for immunohistochemistry. Clinical relevance has not been evaluated.

product information

catalog number :

MBS9203312

products type :

Antibody

products full name :

CLDN2 Antibody (C-term Y224)

products short name :

[CLDN2]

products name syn :

[Claudin-2; SP82; CLDN2]

other names :

[claudin-2; Claudin-2; claudin-2; claudin 2; SP82]

products gene name :

[CLDN2]

other gene names :

[CLDN2; CLDN2]

uniprot entry name :

CLD2_HUMAN

clonality :

Polyclonal

isotype :

host :

Rabbit

reactivity :

Human

sequence length :

230

specificity :

This CLDN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-227 amino acids from the C-terminal region of human CLDN2.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

tested application :

WB, IHC-P, FC, E

app notes :

WB~~1:1000. IHC-P~~1:10~50. FC~~1:10~50

image1 heading :

Anti-CLDN2 A

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Flow Cytometry (FC/FACS)

image4 description :

CLDN2 Antibody (C-term Y224) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

other info1 :

Calculated MW: 24549. Antigen Region: 200-227

other info2 :

Function: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Cellular Location : Cell junction, tight junction. Cell membrane;Multi-pass membrane protein

products categories :

Signal Transduction

products description :

This gene product belongs to the claudin protein familywhose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. This protein is expressed in the intestine. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene.

products references :

Smith, A.J., et al. J. Acquir. Immune Defic. Syndr. 55(3):306-315(2010). Kojima, F., et al. Oncol. Rep. 23(4):927-931(2010). Szakal, D.N., et al. Virchows Arch. 456(3):245-250(2010). Buchert, M., et al. Proc. Natl. Acad. Sci. U.S.A. 107(6):2628-2633(2010). Mankertz, J., et al. Cell Tissue Res. 336(1):67-77(2009)

ncbi gi num :

283806628

ncbi acc num :

NP_001164563.1

ncbi gb acc num :

NM_001171092.1

uniprot acc num :

P57739

ncbi summary :

This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. This protein is expressed in the intestine. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene.[provided by RefSeq, Jan 2010]

uniprot summary :

Claudin-2: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Belongs to the claudin family. Protein type: Membrane protein, multi-pass; Cytoskeletal; Membrane protein, integral. Chromosomal Location of Human Ortholog: Xq22.3-q23. Cellular Component: tight junction; integral to membrane; plasma membrane. Molecular Function: identical protein binding; structural molecule activity. Biological Process: calcium-independent cell-cell adhesion

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage