UGT1A1 Antibody (N-term) | MyBioSource MBS9203265 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

UGT1A1 Antibody (N-term)

catalog :

MBS9203265

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

UGT1A1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane). This demonstrates the UGT1A1 antibody detected the UGT1A1 protein (arrow).

image 2 :

Confocal immunofluorescent analysis of UGT1A1 Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

image 3 :

UGT1A1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of UGT1A1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

product information

catalog number :

MBS9203265

products type :

Antibody

products full name :

UGT1A1 Antibody (N-term)

products short name :

[UGT1A1]

products name syn :

[UDP-glucuronosyltransferase 1-1; UDPGT 1-1; UGT1*1; UGT1-01; UGT11; Bilirubin-specific UDPGT isozyme 1; hUG-BR1; UDP-glucuronosyltransferase 1-A; UGT-1A; UGT1A; UDP-glucuronosyltransferase 1A1; UGT1A1; GNT1; UGT1]

other names :

[UDP-glucuronosyltransferase 1-1; UDP-glucuronosyltransferase 1-1; UDP-glucuronosyltransferase 1-1; UDP glucuronosyltransferase 1 family, polypeptide A1; Bilirubin-specific UDPGT isozyme 1; hUG-BR1; UDP-glucuronosyltransferase 1-A; UGT-1A; UGT1A; UDP-glucuronosyltransferase 1A1]

products gene name :

[UGT1A1]

other gene names :

[UGT1A1; UGT1A1; GNT1; UGT1; UDPGT; UGT1A; HUG-BR1; BILIQTL1; UDPGT 1-1; GNT1; UGT1; UDPGT 1-1; UGT1*1; UGT1-01; UGT1.1; hUG-BR1; UGT-1A; UGT1A]

uniprot entry name :

UD11_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human

sequence positions :

[65-90]

sequence length :

444

specificity :

This UGT1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-90 amino acids from the N-terminal region of human UGT1A1.

purity :

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

app notes :

WB~~1:1000. IF~~1:10~50. IHC-P~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

image3 heading :

Immunohistochemistry (IHC)

other info1 :

Function: UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform glucuronidates bilirubin IX- alpha to form both the IX-alpha-C8 and IX-alpha-C12 monoconjugates and diconjugate. Is also able to catalyze the glucuronidation of 17beta-estradiol, 17alpha-ethinylestradiol, 1-hydroxypyrene, 4- methylumbelliferone, 1-naphthol, paranitrophenol, scopoletin, and umbelliferone. Isoform 2 lacks transferase activity but acts as a negative regulator of isoform 1. Cellular Location: Isoform 1: Microsome. Endoplasmic reticulum membrane; Single-pass membrane protein. Tissue Location: Isoform 1 and isoform 2 are expressed in liver, colon and small intestine. Isoform 2 but not isoform 1 is expressed in kidney. Isoform 1 and isoform 2 are not expressed in esophagus. Not expressed in skin.

products categories :

Metabolism; Signal Transduction

products description :

This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The preferred substrate of this enzyme is bilirubin,although it also has moderate activity with simple phenols, flavones, and C18 steroids. Mutations in this gene result in Crigler-Najjar syndromes types I and II and in Gilbert syndrome.

products references :

Italia, K.Y., et al. Clin. Biochem. 43 (16-17), 1329-1332 (2010) :. Justenhoven, C., et al. Breast Cancer Res. Treat. 124(1):289-292(2010). Hu, M., et al. Pharmacogenet. Genomics 20(10):634-637(2010). Sai, K., et al. Br J Clin Pharmacol 70(2):222-233(2010). Kilic, I., et al. Int J Clin Pharmacol Ther 48(8):504-508(2010)

ncbi gi num :

8850236

ncbi acc num :

NP_000454.1

ncbi gb acc num :

NM_000463.2

uniprot acc num :

P22309

ncbi mol weight :

59591

ncbi pathways :

Pentose And Glucuronate Interconversions Pathway (54658); Pentose And Glucuronate Interconversions Pathway (54658); Pentose And Glucuronate Interconversions Pathway (54658); Pentose And Glucuronate Interconversions Pathway (54658); Pentose And Glucuronate Interconversions Pathway (54658); Pentose And Glucuronate Interconversions Pathway (54658); Pentose And Glucuronate Interconversions Pathway (54658); Pentose And Glucuronate Interconversions Pathway (54658); Pentose And Glucuronate Interconversions Pathway (54658); Pentose And Glucuronate Interconversions Pathway (54658)

ncbi summary :

This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The preferred substrate of this enzyme is bilirubin, although it also has moderate activity with simple phenols, flavones, and C18 steroids. Mutations in this gene result in Crigler-Najjar syndromes types I and II and in Gilbert syndrome. [provided by RefSeq, Jul 2008]

uniprot summary :

UGT1A1: UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform glucuronidates bilirubin IX- alpha to form both the IX-alpha-C8 and IX-alpha-C12 monoconjugates and diconjugate. Is also able to catalyze the glucuronidation of 17beta-estradiol, 17alpha-ethinylestradiol, 1-hydroxypyrene, 4- methylumbelliferone, 1-naphthol, paranitrophenol, scopoletin, and umbelliferone. Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Expressed in liver. Not expressed in skin or kidney. Belongs to the UDP-glycosyltransferase family. 1 isoforms of the human protein are produced by alternative splicing. Protein type: Cofactor and Vitamin Metabolism - retinol; Carbohydrate Metabolism - starch and sucrose; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Lipid Metabolism - androgen and estrogen; Carbohydrate Metabolism - ascorbate and aldarate; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Transferase; Membrane protein, integral; Xenobiotic Metabolism - metabolism by cytochrome P450; Carbohydrate Metabolism - pentose and glucuronate interconversions; Xenobiotic Metabolism - drug metabolism - other enzymes; EC 2.4.1.17. Chromosomal Location of Human Ortholog: 2q37. Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to plasma membrane. Molecular Function: enzyme inhibitor activity; retinoic acid binding; enzyme binding; protein homodimerization activity; glucuronosyltransferase activity; protein heterodimerization activity; steroid binding. Biological Process: steroid metabolic process; response to drug; estrogen metabolic process; organ regeneration; negative regulation of steroid metabolic process; response to lipopolysaccharide; negative regulation of fatty acid metabolic process; liver development; cellular response to hormone stimulus; response to starvation; negative regulation of transferase activity; flavonoid biosynthetic process; bilirubin conjugation; heme catabolic process; xenobiotic metabolic process; digestion; porphyrin metabolic process; negative regulation of catalytic activity; flavone metabolic process; acute-phase response; retinoic acid metabolic process; heterocycle metabolic process; drug metabolic process; response to nutrient. Disease: Gilbert Syndrome; Crigler-najjar Syndrome, Type I; Bilirubin, Serum Level Of, Quantitative Trait Locus 1; Crigler-najjar Syndrome, Type Ii; Hyperbilirubinemia, Transient Familial Neonatal

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage