CYP3A4 Antibody (Center) | MyBioSource MBS9203007 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CYP3A4 Antibody (Center)

catalog :

MBS9203007

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, flow cytometry, FACS, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of lysate from human ovary tissue, using CYP3A4 Antibody (Center). MBS9203007 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

image 2 :

Western blot analysis of lysates from HepG2, A549, HT-29 cell line, human kidney and liver tissue lysate (from left to right), using CYP3A4 Antibody (Center). MBS9203007 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

image 3 :

Western blot analysis of CYP3A4 Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). CYP3A4 (arrow) was detected using the purified Pab.

product information

catalog number :

MBS9203007

products type :

Antibody

products full name :

CYP3A4 Antibody (Center)

products short name :

[CYP3A4]

products name syn :

[Cytochrome P450 3A4; 11413-,8-cineole 2-exo-monooxygenase; Albendazole monooxygenase; Albendazole sulfoxidase; CYPIIIA3; CYPIIIA4; Cytochrome P450 3A3; Cytochrome P450 HLp; Cytochrome P450 NF-25; Cytochrome P450-PCN1; Nifedipine oxidase; Quinine 3-monooxygenase; Taurochenodeoxycholate 6-alpha-hydroxylase; CYP3A4; CYP3A3]

other names :

[cytochrome P450 3A4 isoform 2; Cytochrome P450 3A4; cytochrome P450 3A4; cytochrome P450, family 3, subfamily A, polypeptide 4; 1,8-cineole 2-exo-monooxygenase (EC:1.14.13.157); Albendazole monooxygenase (EC:1.14.13.32); Albendazole sulfoxidase; CYPIIIA3; CYPIIIA4; Cytochrome P450 3A3; Cytochrome P450 HLp; Cytochrome P450 NF-25; Cytochrome P450-PCN1; Nifedipine oxidase; Quinine 3-monooxygenase (EC:1.14.13.67); Taurochenodeoxycholate 6-alpha-hydroxylase (EC:1.14.13.97)]

products gene name :

[CYP3A4]

other gene names :

[CYP3A4; CYP3A4; HLP; CP33; CP34; CYP3A; NF-25; CYP3A3; P450C3; CYPIIIA3; CYPIIIA4; P450PCN1; CYP3A3]

uniprot entry name :

CP3A4_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human

sequence positions :

[228-255]

sequence length :

502

specificity :

This CYP3A4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-255 amino acids from the Central region of human CYP3A4.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS)

app notes :

WB~~1:1000. IHC-P~~1:10~50. FC~~1:10~50

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Western Blot (WB)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

CYP3A4 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYP3A4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

image5 heading :

Flow Cytometry (FC/FACS)

image5 description :

CYP3A4 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

other info1 :

Function: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2-exo-monooxygenase. The enzyme also hydroxylates etoposide (PubMed: 11159812 ). Catalyzes 4-beta-hydroxylation of cholesterol. May catalyze 25-hydroxylation of cholesterol in vitro (PubMed: 21576599 ).

other info2 :

Cellular Location: Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein. Tissue Location: Expressed in prostate and liver. According to some authors, it is not expressed in brain (PubMed:19094056) According to others, weak levels of expression are measured in some brain locations (PubMed:19359404 and PubMed:18545703). Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level).

products categories :

Cardiovascular; Metabolism; Signal Transduction

products description :

CYP3A4, is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs which are are used today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens.

products references :

Sandanaraj,E., Clin. Cancer Res. 14 (21), 7116-7126 (2008). Nelson,D.R., Pharmacogenetics 14 (1), 1-18 (2004). Inoue,K., Jpn. J. Hum. Genet. 37 (2), 133-138 (1992)

ncbi gi num :

322302351

ncbi acc num :

NP_001189784.1

ncbi gb acc num :

NM_001202855.2

uniprot acc num :

P08684

ncbi mol weight :

57343

ncbi pathways :

Phenylalanine Metabolism Pathway (1576); Phenylalanine Metabolism Pathway (1576); Phenylalanine Metabolism Pathway (1576); Phenylalanine Metabolism Pathway (1576); Phenylalanine Metabolism Pathway (1576); Phenylalanine Metabolism Pathway (1576); Phenylalanine Metabolism Pathway (1576); Phenylalanine Metabolism Pathway (1576); Phenylalanine Metabolism Pathway (1576); Phenylalanine Metabolism Pathway (1576)

ncbi summary :

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Feb 2011]

uniprot summary :

CYP3A4: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1 -hydroxylation and midazolam 4- hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2- exo-monooxygenase. The enzyme also hydroxylates etoposide. Belongs to the cytochrome P450 family. Protein type: Xenobiotic Metabolism - drug metabolism - cytochrome P450; Cofactor and Vitamin Metabolism - retinol; EC 1.14.14.1; Xenobiotic Metabolism - drug metabolism - other enzymes; Membrane protein, integral; Oxidoreductase; Lipid Metabolism - linoleic acid; Xenobiotic Metabolism - metabolism by cytochrome P450; Cell surface. Chromosomal Location of Human Ortholog: 7q21.1. Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; cytoplasm; integral to membrane. Molecular Function: enzyme binding; quinine 3-monooxygenase activity; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; heme binding; taurochenodeoxycholate 6alpha-hydroxylase activity; albendazole monooxygenase activity; steroid hydroxylase activity; oxidoreductase activity; oxygen binding; monooxygenase activity; testosterone 6-beta-hydroxylase activity; vitamin D3 25-hydroxylase activity; steroid binding. Biological Process: steroid metabolic process; drug catabolic process; xenobiotic metabolic process; exogenous drug catabolic process; androgen metabolic process; monoterpenoid metabolic process; alkaloid catabolic process; heterocycle metabolic process; lipid metabolic process; drug metabolic process; vitamin D metabolic process; steroid catabolic process

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage