CD45 Antibody (C-term) | MyBioSource MBS9202938 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CD45 Antibody (C-term)

catalog :

MBS9202938

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

The anti-CD45 (C-term) Pab (Cat. # MBS9202938) is used in Western blot to detect CD45 in jurkat cell lysate.

image 2 :

Formalin-fixed and paraffin-embedded human tonsil reacted with CD45 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

image 3 :

Flow cytometric analysis of jurkat cells using CD45 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

product information

catalog number :

MBS9202938

products type :

Antibody

products full name :

CD45 Antibody (C-term)

products short name :

[CD45]

products name syn :

[Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; CD45; PTPRC; CD45]

other names :

[receptor-type tyrosine-protein phosphatase C isoform 1; Receptor-type tyrosine-protein phosphatase C; receptor-type tyrosine-protein phosphatase C; protein tyrosine phosphatase, receptor type, C; Leukocyte common antigen; L-CA; T200; CD_antigen: CD45]

products gene name :

[PTPRC]

other gene names :

[PTPRC; PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180; CD45; L-CA]

uniprot entry name :

PTPRC_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human

sequence positions :

[1245-1275]

sequence length :

1306

specificity :

This CD45 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1245-1275 amino acids from the C-terminal region of human CD45.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

concentration :

Vial Concentration: 2

storage stability :

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

app notes :

WB~~1:1000. IHC-P~~1:10~50. FC~~1:10~50

image1 heading :

Western blot

image2 heading :

Immunohistochemistry

image3 heading :

Flow Cytometry

other info1 :

Antigen Type: Synthetic Peptide. Clone Names: RB00430

other info2 :

Antigen Source: HUMAN. Function: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Cellular Location: Membrane; Single-pass type I membrane protein Membrane raft. Note=Colocalized with DPP4 in membrane rafts

products categories :

Cancer; Immunology; Signal Transduction

products description :

CD45 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. The CD45 gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling.

products references :

Stanton, T., et al., Proc. Natl. Acad. Sci. U.S.A. 100(10):5997-6002 (2003). Vogel, A., et al., Genes Immun. 4(1):79-81 (2003). Rachmilewitz, J., et al., J. Biol. Chem. 278(16):14059-14065 (2003). McCann, F.E., et al., J. Immunol. 170(6):2862-2870 (2003). Fernandis, A.Z., et al., J. Biol. Chem. 278(11):9536-9543 (2003).

ncbi gi num :

392307007

ncbi acc num :

NP_002829.3

ncbi gb acc num :

NM_002838.4

uniprot acc num :

P08575

ncbi mol weight :

147254

ncbi pathways :

Methane Metabolism Pathway (5788); Methane Metabolism Pathway (5788); Methane Metabolism Pathway (5788); Methane Metabolism Pathway (5788); Methane Metabolism Pathway (5788); Methane Metabolism Pathway (5788); Methane Metabolism Pathway (5788); Methane Metabolism Pathway (5788); Methane Metabolism Pathway (5788); Methane Metabolism Pathway (5788)

ncbi summary :

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]

uniprot summary :

CD45: a receptor type protein tyrosine phosphatase protein. Contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. The first PTPAse domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Specifically expressed in hematopoietic cells. An essential regulator of T- and B-cell antigen receptor signaling. Functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. Suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced isoforms have been described. Protein type: Membrane protein, integral; Receptor protein phosphatase, tyrosine; EC 3.1.3.48; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 1q31-q32. Cellular Component: focal adhesion; membrane; integral to plasma membrane; plasma membrane; external side of plasma membrane; lipid raft. Molecular Function: protein binding; transmembrane receptor protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; protein kinase binding. Biological Process: B cell proliferation; axon guidance; substrate-bound cell migration, cell release from substrate; regulation of cell cycle; protein amino acid dephosphorylation; positive regulation of antigen receptor-mediated signaling pathway; T cell receptor signaling pathway; bone marrow development; B cell receptor signaling pathway; stem cell development; cell surface receptor linked signal transduction; dephosphorylation; positive regulation of protein kinase activity; release of sequestered calcium ion into cytosol; negative regulation of T cell mediated cytotoxicity; positive regulation of B cell proliferation; negative regulation of protein kinase activity; positive regulation of T cell proliferation; hemopoietic progenitor cell differentiation; negative regulation of cytokine and chemokine mediated signaling pathway; immunoglobulin biosynthetic process; defense response to virus; T cell differentiation. Disease: Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-positive, Nk Cell-positive; Hepatitis C Virus, Susceptibility To

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage