ALPL Antibody (Center) | MyBioSource MBS9202710 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ALPL Antibody (Center)

catalog :

MBS9202710

quantity :

0.08 mL

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Immunohistochemical analysis ofparaffin-embedded human kidney tissue using MBS9202710 performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

image 2 :

Immunohistochemical analysis of paraffin-embedded human liver tissue using MBS9202710 performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

image 3 :

Anti-ALPL Antibody (Center) at 1:2000 dilution + F9 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

product information

catalog number :

MBS9202710

products type :

Antibody

products full name :

ALPL Antibody (Center)

products short name :

[ALPL]

products name syn :

[Alkaline phosphatase; tissue-nonspecific isozyme; AP-TNAP; TNSALP; Alkaline phosphatase liver/bone/kidney isozyme; ALPL]

other names :

[alkaline phosphatase, tissue-nonspecific isozyme isoform 1; Alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphatase, liver/bone/kidney; Alkaline phosphatase liver/bone/kidney isozyme]

products gene name :

[ALPL]

other gene names :

[ALPL; ALPL; HOPS; TNAP; APTNAP; TNSALP; AP-TNAP; AP-TNAP; TNSALP]

uniprot entry name :

PPBT_HUMAN

clonality :

Polyclonal

isotype :

Rabbit Ig

host :

Rabbit

reactivity :

Human, mouse

sequence positions :

[217-246]

sequence length :

524

specificity :

This ALPL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-246 amino acids from the Central region of human ALPL.

purity :

Purified Rabbit Polyclonal Antibody (Pab)

form :

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

concentration :

Vial Concentration: 1.9

storage stability :

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

tested application :

Flow Cytometry (FC/FACS), ELISA (EIA), ImmunohistochemistrypParaffin (IHC-P-Leica), Western Blot (WB), Immunofluorescence (IF)

app notes :

IHC-P-Leica~~1:500. WB~~1:2000. IF~~1:10~50. FC~~1:10~50

image1 heading :

Immunohistochemical (IHC)

image2 heading :

Immunohistochemical (IHC)

image3 heading :

Western Blot (WB)

image4 heading :

Western Blot (WB)

image4 description :

All lanes : Anti-ALPL Antibody (Center) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: F9 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

image5 heading :

Immunofluorescence

image5 description :

Confocal immunofluorescent analysis of ALPL Antibody (Center)(Cat# MBS9202710) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

other info1 :

Antigen Type: Synthetic Peptide

other info2 :

Antigen Source: HUMAN. Function: This isozyme may play a role in skeletal mineralization. Cellular Location: Cell membrane; Lipid-anchor, GPI-anchor

products categories :

Cancer; Cell Biology; Immunology

products description :

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The genes for the first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. This protein is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization, however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to a disorder known as hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms.

products references :

Panuccio,V., Am. J. Kidney Dis. 50 (6), 1001-1008 (2007). Brun-Heath,I., Eur J Med Genet 50 (5), 367-378 (2007). So,P.P., J. Rheumatol. 34 (6), 1313-1322 (2007)

ncbi gi num :

116734717

ncbi acc num :

NP_000469.3

ncbi gb acc num :

NM_000478.4

uniprot acc num :

P05186

ncbi mol weight :

57305

ncbi summary :

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]

uniprot summary :

ALPL: This isozyme may play a role in skeletal mineralization. Defects in ALPL are a cause of hypophosphatasia (HOPS). HOPS is an inherited metabolic bone disease characterized by defective skeletal mineralization. Four hypophosphatasia forms are distinguished, depending on the age of onset: perinatal, infantile, childhood and adult type. The perinatal form is the most severe and is almost always fatal. Patients with only premature loss of deciduous teeth, but with no bone disease are regarded as having odontohypophosphatasia (odonto). Defects in ALPL are a cause of hypophosphatasia childhood type (HOPSC). Defects in ALPL are a cause of hypophosphatasia infantile type (HOPSI). Belongs to the alkaline phosphatase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.1.3.1; Phosphatase (non-protein); Membrane protein, GPI anchor; Motility/polarity/chemotaxis; Cofactor and Vitamin Metabolism - folate biosynthesis. Chromosomal Location of Human Ortholog: 1p36.12. Cellular Component: extracellular matrix; extracellular space; membrane; plasma membrane; integral to membrane. Molecular Function: protein binding; pyrophosphatase activity; alkaline phosphatase activity; metal ion binding. Biological Process: response to antibiotic; osteoblast differentiation; response to vitamin D; dephosphorylation; response to glucocorticoid stimulus; reproductive developmental process; response to lipopolysaccharide; skeletal development; endochondral ossification. Disease: Hypophosphatasia, Infantile; Hypophosphatasia, Adult; Hypophosphatasia, Childhood

size1 :

0.08 mL

price1 :

165 USD

size2 :

0.4 mL

price2 :

370

more info or order :

MyBioSource product webpage